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Mettl15-Mettl17调节了从早期到晚期前线核糖体过渡的过程

Yury Zgadzay^1,2, Claudio Mirabello³, George Wanes^4,5

¹Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, University of Strasbourg, Illkirch, France.

bioRxiv : the preprint server for biology

|February 3, 2025

概括

甲基转移酶Mettl15和Mettl17合作调节线粒体核糖体组合. 它们在中间阶段的相互作用连接了早期和晚期阶段,确保了小子单元的适当生物发生.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
生物化学生物化学

背景情况:

线粒体核糖体 (线粒体核糖体) 小子单元组装需要rRNA折叠,修饰和蛋白质结合.
组装因子,包括甲基转移酶Mettl15和Mettl17,指导这个复杂的过程.
Mettl17 (早期阶段) 和Mettl15 (后期阶段) 的不同作用及其过渡机制尚未完全理解.

研究的目的:

为了阐明Mettl15和Mettl17在线粒体小子单元组合中合作的机制.
揭示Mettl17和Mettl15之间的相互作用,将早期和晚期组装阶段联系起来.
为了解线粒体生物发生的动态调节提供结构基础.

主要方法:

从 *Trypanosoma brucei* 和哺乳动物同类的结构数据的整合.
分子动力学模拟.分子动力学模拟.
甲基转移酶功能和基质相互作用的生物化学分析.

主要成果:

一个Mettl17作为Mettl15招聘平台的模式.
展示Mettl17释放,使Mettl15形状变化和基质识别成为可能.
甲基化后Mettl15的松结合状态的表征,导致其被启动因子取代.

结论:

Mettl15和Mettl17通过一个连续的招募和释放机制,共同调节线粒体生物发生.
这项研究提供了一个结构性资源,以了解线粒体组装因子的分子适应.
这项工作阐明了小型子单元组装的早期和晚期阶段之间的过渡.

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