细胞外病毒微RNA作为人类细胞中病毒感染的生物标志物
Cheryl Chan1, Joanne Xin Yi Loh1, Wei-Xiang Sin1
1Critical Analytics for Manufacturing Personalized-Medicine, Singapore-MIT Alliance for Research and Technology, Singapore 138602, Singapore.
Molecular therapy. Nucleic acids
|February 3, 2025
概括
细胞外病毒microRNAs (病毒exmiRs) 提供了一种新方法来检测活的,潜伏的和重新激活的病毒感染. 这些无细胞生物标志物通过定量实时逆转录PCR (实时RT-qPCR) 快速和灵敏地检测到.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 核酸放大试验 (NAAT) 检测病毒,但无法区分活体和死体病毒颗粒,也无法区分潜伏和重新激活的感染.
- 目前的诊断方法缺乏确定病毒感染的生存能力或状态的能力.
- 需要敏感和特定的生物标志物来识别病毒活动的不同阶段.
研究的目的:
- 研究细胞外病毒microRNAs (病毒exmiRs) 作为检测活体,潜伏和重新激活病毒感染的无细胞生物标志物.
- 使用定量实时逆转录PCR (实时RT-qPCR) 来确定病毒exmiR检测的灵敏度和速度.
- 为了识别与不同疹病毒及其感染状态相关的特定病毒外表Rs.
主要方法:
- 定量实时逆转录PCR (实时RT-qPCR) 用于敏感检测病毒外型Rs.
- 消耗的细胞培养基和人体血样本被分析,以检测病毒的存在.
- 在埃普斯坦-巴尔病毒 (EBV) 和人类疹病毒8 (HHV-8) 活性化期间,对B细胞模型进行了病毒性exmiR分析.
主要成果:
- 细胞外病毒microRNAs (病毒exmiRs) 被确定为敏感的 (在30天内) 和快速的 (在1天内) 生物标志物.
- 特定的病毒外表R,如EBV miR-BART10-3p和HSV-1 miR-H5,表明T细胞培养中的活感染.
- 细胞外HHV-6 miR-Ro6-4和HCMV miR-US5-2-5p/miR-US22-5p分别作为T细胞和血中潜伏感染的生物标志物.
- 病毒的exmiR签名,包括EBV miR-BHRF1-2-3p和HHV-8 miR-K12家族成员,在病毒重新激活期间升高.
结论:
- 细胞外病毒microRNAs (病毒exmiRs) 显示出作为诊断活体,潜伏和重新激活病毒感染的无细胞生物标志物的显著潜力.
- 病毒的exmiR可以通过提供关于病毒活动和生存能力的关键信息来补充NAAT.
- 这项研究强调了病毒外表在促进复杂病毒感染的检测和理解方面的实用性.
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