由微生物罗多素产生的质子动力促进了细胞外电子转移
Wenqi Ding1, Tong Lin2, Yun Yang3
1Frontiers Science Centre for Synthetic Biology (Ministry of Education), And Key Laboratory of Systems Bioengineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Synthetic and systems biotechnology
|February 3, 2025
概括
微生物罗多普辛增强了电活性细菌中的细胞外电子转移 (EET) 效率. 在Shewanella oneidensis中,Rhodopsin cR-1的异质表达通过增加质子动力 (PMF) 来显著提高功率输出.
科学领域:
- 生物电化学 生物电化学
- 微生物生理学 微生物生理学
- 合成生物学 合成生物学
背景情况:
- 低的细胞外电子转移 (EET) 效率限制了生物电化学系统中的电活性微生物.
- 质子驱动力 (PMF) 是细菌中一个关键的能量传输途径,但它对ETE的直接影响尚不清楚.
- 微生物罗多普辛为非光合作用细胞利用PMF提供了一个潜在的机制.
研究的目的:
- 研究微生物罗多普辛 (Arch,Mac,cR-1) 在提高ETE效率中的作用.
- 为了评估这些罗多素在*Shewanella oneidensis*中的异质表达.
- 阐明改进ETT和发电的基本机制.
主要方法:
- 在S. oneidensis*中,三种微生物罗多素 (Arch,Mac,cR-1) 的异质表达.
- 在重组菌株中测量输出功率密度.
- 在cR-1表达菌株中分析能量和物质代谢的转录学分析.
主要成果:
- 表达罗多素cR-1的重组菌株实现了最高的输出功率密度 (0.87W/m2),比野生类型S.oneidensisMR-1增加了3.49倍.
- 罗多普辛cR-1作为光驱动的质子,增加PMF和细胞ATP供应.
- 增强的PMF促进乳酸的吸收和利用,促进细胞内电子生成和EET速率.
结论:
- 微生物罗多素cR-1的异质表达显著提高了S.oneidensis*的ETE效率和发电.
- 该机制涉及光驱动的质子,增加PMF和改善基质代谢.
- 微生物罗多普辛是优化生物电化学系统的一个有希望的策略.
相关概念视频
Electron Transport Chain: Complex III and IV
6.9K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
6.9K
The Supercomplexes in the Crista Membrane
2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Channel Rhodopsins
2.5K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.5K
The Z-Scheme of Electron Transport in Photosynthesis
9.8K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
9.8K
Electron Transport Chains
97.0K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
97.0K
Photosystem I
61.6K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
61.6K


