双边细胞流在胚胎胃化中,在左右组织者形成之前表现出不对称性
Rieko Asai1,2, Shubham Sinha3, Vivek N Prakash3,4,5
1Cardiovascular Research Institute, University of California, San Francisco, CA 94158.
概括
早期胚胎发育显示左右不对称 (LR) 通过反旋转的细胞运动在中线形成之前. 这一物理过程在关键发育结构之前,影响了羊胃的LR横向性.
科学领域:
- 发展生物学 发展生物学
- 生物物理学的生物物理.
- 胚胎学 胚胎学
背景情况:
- 双边的身体计划在动物中占主导地位.
- 左-右 (LR) 非对称模式遵循胚胎胚胎中的中线形态发生.
- 在中线形态发生之前LR不对称的时间和机制仍然不清楚.
研究的目的:
- 为了研究在早期的胎儿胃化过程中LR不对称的启动和机制.
- 探索细胞运动在中线结构形成之前建立LR不对称性的作用.
主要方法:
- 在小胚胎模型系统中细胞流量的生物物理量化.
- 细胞运动动态的高空间和时间分辨率分析.
- 研究了线粒体停止对细胞流和LR不对称性的影响.
主要成果:
- 大规模的双边反旋转细胞流,称为"波罗纳斯运动",在早期胃流中表现出LR不对称性.
- 这些运动先于原始线条和亨森节点的形成.
- 在6小时后观察到双边流动中的右翼主导地位,即使在原始连续形成减少后也持续存在.
结论:
- 胚胎胃口中的LR不对称性比以前的模型更早地检测到,这些模型专注于亨森节点的横向性基因.
- 物理过程,如细胞流动力学,在影响胚胎发育期间LR侧向性方面发挥着重要作用.
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