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设计近红外光诱导的功能上转化纳米颗粒作为支持酶不动化的设计:增强生物催化剂活性和稳定性

Ahmet Ulu¹, Samir Abbas Ali Noma², Ali Kuruçay¹

¹Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, İnönü University, 44280 Malatya, Türkiye.

International journal of biological macromolecules

|February 3, 2025

概括

向上转换的纳米粒子 (UCNP) 可以通过Förster共振能量转移在近红外光下激活PEG-L-ASNase (P-Lase). 这种新的方法增强了P-酶的活性,为白血病治疗提供了潜在的可能性.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
生物化学生物化学

背景情况:

阿斯巴拉金酶对于白血病治疗至关重要,但患有稳定性问题.
上转化纳米粒子 (UCNPs) 为生物医学应用提供了独特的光学特性.
近红外 (NIR) 辐射可以远程激活治疗剂.

研究的目的:

通过Förster共振能量转移 (FRET) 调查通过UCNP介导的PEG-L-ASNase (P-Lase) 的激活.
通过在功能化的UCNP上固定,增强P-Lase的稳定性和活性.
探索这个系统在白血病治疗中的潜力.

主要方法:

将P-酶固定到功能化的NaYF4:Yb3+,Er3+,Nd3+UCNP上.
固定酶的稳定性 (pH,热,溶剂,储存) 的表征.
通过体外细胞毒性和血液溶解试验评估UCNP生物相容性.
在NIR照射下测量P-激酶活性增强.

主要成果:

固定的NaYF4:Yb3+,Er3+,Nd3+/GPTMS-P-Lase表现出卓越的pH,热和储存稳定性.
在多个循环和在不同温度下长时间储存后,酶保持了显著的活性.
在体外试验证实了合成的UCNP的生物相容性.
尼尔射线照射导致P-激酶活性增加4倍以上.

结论:

UCNP固定显著改善P-Lase的稳定性,并保持其活动.
通过FRET在NIR照射下,UCNP发射可以有效地触发P-Lase活动.
这种方法为提高酶催化效率提供了一种新的策略,在白血病治疗中具有潜在的应用.

关键词:

在L-阿斯巴拉基纳酶的固定过程中.接近红外的近红外.表面的修改是表面的修改.触发活动活动.向上转换纳米粒子

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