相关实验视频

Updated: May 29, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

通过牛津纳米孔长读测序识别循环RNA变异

Chloé Bessière^1,2,3, Fabienne Meggetto^1,2, Christine Gaspin^4,5

¹Centre de Recherches en Cancérologie de Toulouse (CRCT), UMR-1037 INSERM, UMR-5071 CNRS, Université Toulouse 3, Toulouse, France.

Methods in molecular biology (Clifton, N.J.)

|February 3, 2025

概括

本研究提出了一种使用牛津纳米孔技术测序全长圆形RNA (circRNAs) 的新协议. 这种方法有助于理解circRNA功能和在各种疾病背景下进行替代拼接.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
在RNA生物学,RNA生物学.

背景情况:

循环RNA (circRNAs) 是一类参与基因表达调节的非编码RNA.
确定circRNAs的完整序列对于理解它们的功能至关重要,但仍然具有挑战性.
现有的检测方法往往难以提供完整的序列信息.

研究的目的:

描述一种用于丰富circRNAs的协议,用于长读测序.
使用牛津纳米孔平台实现circRNAs的全长测序.
为高可靠性circRNA检测提供生物信息学指导.

主要方法:

循环RNAs的丰富.
使用牛津纳米孔平台进行长读测序.
使用两个不同的计算工具进行生物信息分析.

主要成果:

成功完成了circRNAs的全长测序.
在circRNAs中检测替代拼接事件.
识别高可信度的环RNAs.

结论:

开发的协议有助于进行全面的circRNA分析.
这种方法适用于各种疾病研究领域.
它增强了对circRNA功能和基因调节中的作用的理解.

关键词:

这是循环RNARNA.长读序列的测序方式没有编码的RNA.牛津的纳米孔拼接拼接拼接拼接

更多相关视频

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...