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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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结合短阅读和长阅读的转录组,以进行向的酶发现.

Mojca Juteršek1, Marko Petek2, Špela Baebler2

  • 1National Institute of Biology, Department of Biotechnology and Systems Biology, Ljubljana, Slovenia. Mojca.Jutersek@nib.si.

Methods in molecular biology (Clifton, N.J.)
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概括

这项研究提出了一个转录组学管道,用于识别在非模型生物体中具有特定酶活性的基因. 该方法结合了多样化的测序数据,用于准确的基因发现,帮助生物技术和代谢途径研究.

关键词:
从新开始,转录组组合组合.酶识别 酶识别这是Iso-Seq.文字转录学 (Transcriptomics) 是一个学科.

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科学领域:

  • 生命科学 生命科学
  • 生物技术是生物技术.
  • 基因组学就是基因组学.

背景情况:

  • 鉴定特定酶活性的基因对于生命科学和生物技术至关重要.
  • 许多参与二次代谢产生的基因和专门的代谢途径仍然未被发现.
  • 由于大自然的多样性,需要使用高吞吐量方法来发现基因,事先的知识是最小的.

研究的目的:

  • 描述一个转录组学管道,用于发现在非模型物种中编码特定酶活性的候选基因.
  • 提供一种使用组合测序数据的向基因发现方法.
  • 为各种生物问题提供详细的分析和质量控制指令.

主要方法:

  • 生成和整合短读和长读的转录基因数据.
  • 获得了全长的转录序列,等位基变异和异构体表达数据.
  • 利用蛋白质域注释和转录基因数据来选择候选基因进行活性测试.

主要成果:

  • 开发了一个强大的转录学管道,用于识别具有酶活性的基因.
  • 成功选择候选基因进行进一步的功能验证.
  • 该管道提供可靠的全长转录序列和表达信息.

结论:

  • 描述的转录组学管道有效地发现了在非模型生物体中编码特定酶活性的基因.
  • 这种方法有助于识别用于生物技术应用的新基因.
  • 管道的详细说明和质量控制步骤提高了其适用于各种研究领域的应用性.