相关实验视频

Updated: May 29, 2025

A Murine Model of Muscle Training by Neuromuscular Electrical Stimulation

A Murine Model of Muscle Training by Neuromuscular Electrical Stimulation

Published on: May 9, 2012

hBMSC-EVs通过抑制氧化应激和炎症来减轻无重感诱导的骨肌缩

Mengyuan Chang¹, Ruiqi Liu², Bingqian Chen³

¹Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.

Stem cell research & therapy

|February 3, 2025

概括

人类骨髓介质干细胞衍生的细胞外囊泡 (hBMSC-EVs) 在对抗因不使用和微重力造成的肌肉缩方面表现有前途. 这些囊泡通过减少氧化应激,炎症和蛋白质降解途径来防止肌肉损失.

科学领域:

生物医学工程生物医学工程
再生医学是一种再生医学.
太空医学太空医学

背景情况:

太空飞行引起的肌肉缩是一个重大问题,除了运动之外没有有效的治疗方法.
骨髓中酶干细胞衍生的细胞外囊泡 (BMSC-EVs) 具有抗炎和抗氧化特性.
BMSC-EVs为缓解无重量肌肉缩提供了一个潜在的治疗策略.

研究的目的:

研究人类BMSC-EVs (hBMSC-EVs) 在预防和逆转肌肉缩方面的疗效.
阐明hBMSC-EVs对骨肌肉施加保护作用的分子机制.

主要方法:

对hBMSC-EVs的隔离和表征.
建立C2C12神经管 (营养剥夺) 和老鼠尾部悬浮模型.
评估肌肉质量,纤维横截面积,握力和分子标记 (ROS,炎症,UPS,自).

主要成果:

在小鼠中,hBMSC-EVs有效降低了肌管缩,并逆转了无重量诱导的肌肉质量,CSA和握力减少.
hBMSC-EVs降低了与基蛋白酶体系统 (UPS) 和氧化应激相关的蛋白质水平.
通过上调Sirt1和PGC-1α,hBMSC-EVs抑制了UPS和自-溶酶体通路,抑制了氧化应激和炎症,并保留了肌肉纤维类型.

结论:

通过抑制UPS,自,氧化应激和炎症,hBMSC-EVs有效抑制肌肉缩.
在无重量模型中,hBMSC-EVs可以逆转肌肉纤维类型的转换,并增强肌肉功能.
这些发现表明hBMSC-EVs对肌肉缩的保护潜力,并进一步了解其分子机制.

关键词:

骨髓中介细胞干细胞是骨髓中介细胞的干细胞.细胞外囊泡中的细胞外囊泡.炎症炎症是一种炎症.肌肉缩肌肉缩肌肉缩氧化应激是一种氧化应激.

更多相关视频

Paradigms of Lower Extremity Electrical Stimulation Training After Spinal Cord Injury

Paradigms of Lower Extremity Electrical Stimulation Training After Spinal Cord Injury

Published on: February 1, 2018

Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles

Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles

Published on: February 7, 2025

相关概念视频

Myasthenia Gravis: Overview and Treatment

Myasthenia Gravis: Overview and Treatment

Myasthenia gravis is a neuromuscular transmission disorder characterized by weakness and increased fatigability of skeletal muscles. It is an autoimmune disease affecting approximately one in 2000 people, where antibodies against the α1 subunit of nicotinic acetylcholine receptors are produced.
These antibodies interfere with the function of the nicotinic receptors in three ways: by binding to the receptor and disrupting acetylcholine binding; by causing cross-linking of receptors which...

Satellite Stem Cells and Muscular Dystrophy

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...