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相关概念视频

Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

7.4K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
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相关实验视频

Updated: May 29, 2025

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
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A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

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组织清除及其在纳米粒子开发中的应用.

Zhouyi Rong1,2, Ali Ertürk1,2,3,4, Yamei Tang5

  • 1Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, 85764, Neuherberg, Germany.

Small (Weinheim an der Bergstrasse, Germany)
|February 4, 2025
PubMed
概括
此摘要是机器生成的。

组织清除技术使整个生物体中纳米粒子的3D可视化成为可能,这对于理解纳米粒子生物分布和开发更安全,更有效的纳米药物至关重要.

关键词:
在3D成像中使用3D成像.光片光显微镜光片光显微镜纳米颗粒是一种纳米粒子.组织清除 组织清除

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Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing
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Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

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ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
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ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging

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相关实验视频

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A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

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Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing
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科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术 纳米技术
  • 细胞成像 细胞成像

背景情况:

  • 纳米粒子为生物医学应用提供独特的特性,例如药物输送和诊断.
  • 在整个生物体中评估纳米粒子在细胞水平上的生物分布对于安全性和有效性至关重要.
  • 现有的成像方法 (MRI,CT) 和组织切片缺乏必要的分辨率或系统视图.

研究的目的:

  • 审查纳米粒子生物分布研究的组织清除技术的进展.
  • 突出组织清理在生物系统中纳米粒子3D可视化中的潜力.
  • 为开发基于纳米粒子的疗法提供见解.

主要方法:

  • 探索各种组织清除方法.
  • 组织清除的应用用于研究纳米粒子生物分布.
  • 清除组织的3D成像和分析.

主要成果:

  • 组织清除使整个器官和整个生物体的细胞水平成像成为可能.
  • 这些方法克服了传统成像和切割技术的局限性.
  • 促进对纳米粒子分布和生物系统内的相互作用的全面理解.

结论:

  • 组织清除是可视化纳米粒子生物分布的强大工具.
  • 组织清除方面的进步对于未来的纳米医学发展至关重要.
  • 这种方法增强了对纳米粒子安全性和治疗潜力的评估.