含二氧化素的银纳米粒子通过调节氧化应激和炎症通路来缓解B[a]P诱导的肺纤维化
Ahmed Salah1, Maiven M Edward2, Mohammed A Hussein3
1Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia, Egypt
Pharmaceutical nanotechnology
|February 4, 2025
概括
载有二氧化原蛋白的银纳米粒子 (Dio-AgNPs) 通过减少炎症和氧化应激,有效地对抗小鼠的[a]烯诱导的肺纤维化. 这种纳米医学方法增强了diosgenin.
科学领域:
- 纳米医学是一种纳米医学.
- 呼吸系统医学 呼吸系统医学
- 毒理学 毒理学 毒理学
背景情况:
- 肺纤维化,肺组织的痕,通常是由诸如佐[a]烯 (B[a]P) 这样的毒素引起的.
- 迪奥斯基宁是一种天然化合物,具有抗炎和抗氧化特性,但具有不良的溶解性和生物可用性.
- 开发有效的迪奥斯基宁输送系统对于治疗肺损伤至关重要.
研究的目的:
- 开发和表征素载银纳米粒子 (Dio-AgNPs).
- 在小鼠模型中研究Dio-AgNPs对B[a]P诱导的肺纤维化的保护作用.
- 评估Dio-AgNPs对纤维化关键分子和遗传标记物的影响.
主要方法:
- Dio-AgNPs的合成和表征,包括大小,泽塔潜力和封装效率.
- 在小鼠中使用B[a]P暴露诱导肺纤维化.
- 管理Dio-AgNPs和生物化学标记 (脂质,氧化应激,炎症) 和基因表达 (STAT3,TGF-β1,SIRT1) 的评估.
- 在基分子对接中预测迪奥斯基宁与蛋白的结合亲和力.
主要成果:
- 通过成功合成具有最佳特性的Dio-AgNPs (51.60±1.54纳米直径,19.5mV的泽塔电位,84.98%的封装效率).
- 暴露于B[a]P诱导了纤维化迹象的显著变化,包括改变的脂质样本,增加的氧化应激和炎症标志物,以及失调的基因表达.
- 迪奥-AgNPs的使用逆转了这些有害变化,显著改善了生物化学标记物,并使基因表达正常化.
- 分子对接证实了迪奥斯基宁与SIRT1,STAT3和TGF-β1.1之间的强结合相互作用.
结论:
- 迪奥-AgNPs代表了一个创新的和有效的纳米载体的diosgenin,克服其生物可用性限制.
- 迪奥-AgNPs通过减少氧化应激和炎症来减轻B[a]P诱导的肺纤维化具有显著的治疗潜力.
- 通过分子对接数据支持的STAT3,TGF-β1和SIRT1通路的调节,突显了Dio-AgNPs在呼吸系统疾病中的作用机制和治疗前景.
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