相关实验视频
Updated: May 29, 2025
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
12.1K
循环RNA在血管细胞分化过程中增加,是血管疾病的生物标志物
Bernd H Northoff1, Andreas Herbst1, Catharina Wenk1
1Institute of Laboratory Medicine, LMU University Hospital, LMU Munich, Marchioninistr. 15, 81377 Munich, Germany.
Cardiovascular research
|February 4, 2025
概括
循环RNAs (circRNAs) 在血管细胞发育过程中增加,但在动脉样硬化中减少. 特定的circRNA显示出作为检测动脉样硬化血管疾病的血液生物标志物的潜力.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 干细胞生物学 干细胞生物学
背景情况:
- 循环RNAs (circRNAs) 是一种RNA分子类,在基因调节中的作用尚未完全理解.
- 它们在细胞分化过程中的动态表达和作为疾病生物标志物的潜力需要进行系统的研究.
研究的目的:
- 在人类诱导的多能干细胞 (iPSC) 分化成血管内皮细胞 (EC) 和光滑肌细胞 (SMC) 期间调查circRNAs的时间表达.
- 探索已识别的circRNAs作为人类血管疾病,特别是动脉样硬化的生物标志物的潜力.
主要方法:
- 高通量RNA测序和de novocircRNA检测管道被用于在2周的iPSC分化过程中量化circRNA水平.
- 机器学习模型,包括接收器操作特征 (ROC) 曲线分析,用于评估患者样本中circRNAs的诊断潜力.
主要成果:
- 在EC和SMC分化过程中观察到circRNA水平的全球增加,与宿主基因上调和剪接因子下调有关.
- 在血管细胞成熟期间,MYC转录因子水平下降,影响拼接因子和circRNA表达.
- 与差异化相反,患者的动脉样硬化组织和外周血液单核细胞 (PBMC) 中的circRNA水平下降.
- 一个由circRNAs (COL4A1,COL4A2,HSPG2,YPEL2) 组成的小组从健康对照组中区分了动脉样硬化组织 (AUC=0.79) 和PBMCs (AUC=0.73).
结论:
- 时间解析的转录基因分析显示,circRNAs为疾病分析提供了详细的分子洞察力.
- 在这项研究中确定了特定的circRNAs,作为用于检测动脉样硬化血管疾病的非侵入性血基生物标志物具有前途.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
02:15Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
03:14Notch Signaling Pathway
4.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K
01:22MicroRNAs
21.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K