相关实验视频
Updated: May 29, 2025

10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
35.7K
BRD9通过激活MAPK/ERK通路来促进甲状腺癌的恶性表型
Yingcheng Deng1, Yilin Li2, Hong Cao2
1Department of Anatomy, Hunan Traditional Chinese Medical College, Zhuzhou.
Anti-cancer drugs
|February 4, 2025
概括
含原蛋白9 (BRD9) 的高表达驱动甲状腺癌恶性. 抑制BRD9或MAPK/ERK通路可降低癌症生长,促进细胞亡,提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 甲状腺癌是一种普遍存在的内分泌恶性瘤.
- 包括BRD9在内的基因组和外端域 (BET) 蛋白质对于表观基因组解释至关重要.
- BRD9在甲状腺癌发病过程中的特定作用在很大程度上仍未被探索.
研究的目的:
- 研究BRD9在甲状腺癌中的作用.
- 为了确定BRD9调制对甲状腺癌细胞行为的影响.
- 为了阐明参与BRD9-介导的甲状腺癌进展的信号通路.
主要方法:
- 在甲状腺癌细胞系和患者组织中评估BRD9表达.
- 使用BRD9敲击和过度表达技术.
- 使用的药理抑制剂,包括I-BRD9和SCH772984 (ERK抑制剂).
- 分析了MAPK/ERK信号通路内的蛋白质的表达.
主要成果:
- 高的BRD9表达与甲状腺癌中更恶性的表型相关.
- 抑制BRD9抑制了繁殖并诱导了细胞灭绝;I-BRD9治疗显示了类似的效果.
- BRD9促进异种移植瘤的生长.
- BRD9激活了MAPK/ERK通路,通过改变Raf,ERK,p-ERK,c-Fos和c-Myc的表达来证明这一点.
- 抑制ERK可以逆转BRD9诱导的通路激活.
结论:
- 在甲状腺癌患者的血清和瘤组织中,BRD9的表达很高.
- 通过激活MAPK/ERK信号通路,BRD9促进了甲状腺癌的进展.
- 向BRD9或MAPK/ERK通路为甲状腺癌提供了潜在的治疗策略.
更多相关视频
相关概念视频
MAPK Signaling Cascades
5.1K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.1K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Receptor Tyrosine Kinases
12.2K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
12.2K

