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相关概念视频

Reporter Genes02:11

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Fluorescence detection methods for microfluidic droplet platforms
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聚合诱导的基于排放的光探针用于细胞微环境检测.

Xiang-Fei Li1, Fu-Gen Wu1

  • 1State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, 2 Southeast University Road, Nanjing, 211189, China.

Biosensors & bioelectronics
|February 4, 2025
PubMed
概括
此摘要是机器生成的。

聚合诱导发射 (AIE) 光分子为细胞微环境提供先进的成像. 这篇评论强调了他们在检测pH,粘度和生物分子等关键参数方面的进展,以了解疾病.

关键词:
聚合引发的排放量 聚合引发的排放量细胞成像 细胞成像细胞微环境是细胞微环境.检测 检测 检测 检测 检测光探测器的光探测器

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科学领域:

  • 生物医学工程 生物医学工程
  • 化学生物学 化学生物学
  • 分子成像学分子成像学

背景情况:

  • 细胞微环境极大地影响细胞行为和疾病的发病.
  • 微环境的动态监测有助于疾病风险预测和疾病进展跟踪.
  • 异常的细胞微环境与各种疾病有关.

研究的目的:

  • 审查最近使用聚合诱导排放 (AIE) 光分子用于细胞微环境检测的进展.
  • 突出AIE探针在成像和感应关键微环境参数和生物组件中的应用.
  • 讨论AIE探测器的设计原则,机制,性能和未来前景.

主要方法:

  • 专注于AIE的光探测器来感知细胞微环境的参数.
  • 对检测pH,粘度,极性,温度,气体分子,金属离子,氧化还原状态和蛋白质的探针进行分析.
  • 审查探头设计,检测机制,传感性能和生物应用.

主要成果:

  • AIE分子表现出独特的特征,有利于细胞微环境成像和传感.
  • 在开发各种微环境分析物的AIE探针方面取得了重大进展.
  • AIE探测器可以精确检测细胞环境中的关键参数和生物成分.

结论:

  • AIE光探测器是研究细胞微环境的强大工具.
  • 持续开发AIE探针有望提高生物检测的灵敏度和特异性.
  • 这一领域对推进疾病诊断和理解生物过程具有重大潜力.