确定关键的circ-mi-mRNA相互作用网络,调节丸发育和精子生成
Xiaopeng Li1, Xiaoyong He1, Guibi Li1
1State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory of Livestock and Poultry Multi-omics Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, PR China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
亚精子种子表现出丸异常和改变的基因表达. 循环RNAs (circRNAs),微RNAs (miRNAs) 和信使RNAs (mRNAs) 是调节 testicular 发育和精液质量的关键.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 动物科学动物科学
背景情况:
- 精液质量对于的繁殖成功至关重要.
- 在甘中观察到亚精子,但了解得很少.
- 丸形态和分子机制需要研究.
研究的目的:
- 为了比较丸形态和组织学之间的normozoospermic和亚精子.
- 分析子丸中circRNAs,miRNAs和mRNAs的差异表达.
- 阐明ceRNAs在子精子生成和精液质量中的调节作用.
主要方法:
- 体丸的历史形态学和组织学分析.
- 全转录组测序 (RNA-seq) 用于circRNA,miRNA和mRNA分析.
- 生物信息分析包括功能丰富和ceRNA网络建设.
主要成果:
- 与常态动物精子相比,亚精子的丸重量和直径显著降低,组织学参数发生变化.
- 683个DEcircRNA,24个DEmiRNA和1118个DEG在各组之间被确定.
- 发现关键的circRNAs通过miRNAs调节TGFB2和BCL2的表达,影响丸细胞的亡,增殖和精子生成.
结论:
- 甘德精症与丸异常有关,而不是体重.
- 环RNAs,miRNAs和mRNAs的差异表达在子丸发育中起着至关重要的作用.
- 涉及特定circRNA和miRNA的ceRNA网络对于调节子精子生成和精液质量至关重要.
相关概念视频
MicroRNAs
Regulation of Expression Occurs at Multiple Steps
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The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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