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在草鱼中的潜在地质素结合蛋白的识别,基于亲和度响应目标稳定性和双重质量标签蛋白学

Jun-Ming Zhang¹, Huan Han¹, Bing Fu²

¹China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China.

Ecotoxicology and environmental safety

|February 4, 2025

概括

研究人员在草鱼中发现了关键蛋白质,这些蛋白质与有机无味污染物地球结合. 这一发现提高了对污染物如何影响水生生物的理解,并有助于环境风险评估.

科学领域:

环境科学环境科学
水生毒理学水生毒理学
分子生物学分子生物学

背景情况:

由于有机无味化合物 (如地胺) 的水污染影响了水产品的质量和消费者接受度.
目前对地质矿物质的研究重点是生态毒性,对其在水生生物体内的分子相互作用的理解有限.
识别地质素结合蛋白对于理解其在分子水平上的生物效应至关重要.

研究的目的:

在草鱼 (Ctenopharyngodon idella) 的组织中识别地质素结合蛋白.
开发和验证一种综合方法,结合体外,体内和体内的污染物标识方法.
推进对污染物与生物相互作用的理解,以改善环境风险评估.

主要方法:

在体外进行亲和反应性目标稳定性 (ARTS) 试验,以确定潜在的结合蛋白.
协同质量标记 (TMT) 在体内蛋白质组学,以分析暴露于地质胺的鱼类中的蛋白质表达变化.
分子对接模拟用于预测已识别的蛋白质与geosmin的结合亲和力.

主要成果:

在ARTS分析中,发现了56种候选蛋白质,其中膜相关蛋白质如catenin beta-1和annexin.
蛋白质组分析显示,在对地质暴露的反应中,有256种不同表达的蛋白质.
在体外和体内实验方法中,共有18种蛋白质,其中附素,甲素D和互白素-1受体被突出显示为潜在的地质素标. 安素在中显示出最高的结合亲和力.

结论:

该研究成功地在草鱼状组织中使用综合的多omics方法识别了潜在的地质素结合蛋白.
素,甲素D和互乐金-1受体被提议作为地质的关键分子标.
开发的协议为调查污染物-蛋白质相互作用提供了一个强大的框架,增强环境风险评估策略.

关键词:

亲密关系 (Affinity) 是一种关系.结合蛋白质是一种结合蛋白质.分子对接是分子对接.没有风味的化合物.有机污染物有机污染物污染物-蛋白质相互作用

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