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建模和典型案例分析细胞规模的跨膜运输和细胞内结晶和再结晶在冷解过程中

Pengsong Yuan¹, Xueqiang Dong¹, Haocheng Wang²

¹Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

|February 4, 2025

概括

这项研究开发了一个冷保存的数值模型,找到最佳的冷却速率和温度,以最大限度地减少小鼠卵细胞中的冰晶损伤,并改善细胞恢复.

科学领域:

低温生物学低温生物学
生物物理学的生物物理.
计算生物学计算生物学

背景情况:

在冷解周期期间形成冰晶会造成机械和溶液损伤,影响生物样本的生存能力.
了解细胞内冰结晶和水/冷保护剂 (CPA) 运输对于有效的冷保存至关重要.

研究的目的:

开发一个全面的数值模型,模拟冷保存过程.
确定最佳的冷却和回暖速度,以最大限度地减少对生物样本,特别是小鼠卵细胞的损害.
为改善实际的冷保存协议提供数值指导.

主要方法:

构建了一个数字模型,包括结晶,再结晶和跨膜运输动力学.
该模型模拟了不同冷却和回暖速度对细胞内冰体积和CPA度的影响.
参数扫描确定了冷却速度和初始DMSO度的有利范围,以恢复小鼠卵细胞.

主要成果:

较高的冷却速度增加了细胞内冰体积和核化温度.
发现0.5-1°C·min-1的冷却速度对小鼠卵细胞造成了最小的伤害.
更快的回暖速度降低了细胞内冰体积;建议进行预再结晶脉冲.
低于-160°C的冷保存,运行90秒以下,可以在处理过程中最大限度地减少再结晶.
最佳回收条件包括0.4-1.8°C·min-1和0.1-0.3 M DMSO.的冷却速度.

结论:

数值模型根据物理参数准确预测了冷保存结果.
特定的冷却速率和温度对于保持小鼠卵细胞生存能力至关重要.
该模型为优化生物标本冷保存策略提供了有价值的定量见解.

关键词:

结解的过程.细胞内冰体积的细胞内冰体积.溶解物中的度.通过膜的运输.

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