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干燥作为一种合适的替代品冷藏的植物圈样本基于DNA的微生物社区分析的DNA分析

Emily Smenderovac^1,2, Karelle Rheault³, Marie-Ange Moisan⁴

¹Great Lakes Forestry Centre, Natural Resources Canada, ontario, Canada. emily.smenderovac@nrcan-rncan.gc.ca.

Scientific reports

|February 5, 2025

概括

凝包装有效地在环境温度下保护植物植物界微生物群落,用于短期储存. 这种方法允许基于DNA的可靠分析,区分树种和采样地点.

科学领域:

微生物学微生物学
生态生态学生态生态学
分子生物学分子生物学

背景情况:

保护植物植物界微生物群落用于DNA分析在偏远的现场环境中具有挑战性.
传统的冷方法并不总是可行的,需要使用替代的环境温度保存技术.

研究的目的:

评估干燥 (凝包) 和保存溶液 (乙醇,RNAlater,LifeGuard) 在环境温度下对植物圈微生物社区DNA保存的有效性.
评估这些方法在三周内对DNA质量和社区组成的影响.
为了确定干燥是否保留了树种和采样地点之间的区分能力.

主要方法:

来自Populus tremuloides和Picea glauca叶的菌微生物群体使用凝包,95%乙醇,RNAlater和LifeGuard在21°C下保存长达三周.
分析了DNA度和质量.
细菌和真菌群体使用metabarcoding进行了分析.
一项对Populus grandidentata的二次研究测试了干燥能够解决采样地点差异的能力.

主要成果:

凝包装在环境温度下有效地保护了植物界的细菌和真菌群落.
虽然在凝保存过程中发生了一些社区变化,但这些并没有妨碍区分树种或采样地点的能力.
乙醇,RNAlater和LifeGuard显示出不同程度的疗效,而凝成为可靠的选择.

结论:

使用凝包的干燥是一种可行且有效的方法,用于基于DNA的微生物社区研究,在环境温度下短期保存植物圈样本.
该方法支持在资源有限或偏远环境中的生态研究.
凝保存维护了物种和地点歧视的社区数据的完整性.

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