相关实验视频

Updated: May 29, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

原生动物的纳米孔测序:将生物化学分子链上的生物信息解码为人类可读的信号

Branden Hunter¹, Timothy Cromwell², Hyunjin Shim^1,3

¹Department of Biology, California State University, 2555 East San Ramon Ave, Fresno, CA 93740, USA.

Computational and structural biotechnology journal

|February 5, 2025

概括

纳米孔测序有效地解码元基因组样本中的复杂基因组. 这项长期阅读的技术准确地识别和组装了感染小鼠血液中的Trypanosoma brucei DNA,显示了寄生虫基因组学的前景.

科学领域:

基因组学就是基因组学.
生物信息学是一种生物信息学.
寄生虫学的寄生虫学

背景情况:

生物信息编码在分子序列中,就像核酸一样.
纳米孔测序为连续的基因组信息提供了长读数,有助于复杂区域分析.
大基因组样本对传统的短读测序构成挑战.

研究的目的:

评估纳米孔测序,以解码在元基因组样本中的独特基因组.
评估纳米孔测序在分类和组装超变性基因组中的有效性.
研究纳米孔测序用于Trypanosoma brucei在感染小鼠中的检测.

主要方法:

使用纳米孔技术对被Trypanosoma brucei感染的小鼠的血液和便样本进行测序.
纳米孔的分类学分类可以识别T. brucei的存在.
纳米孔的metagenomic de novo组装可以重建T. brucei基因组片段.

主要成果:

在受感染的小鼠血液样本中发现了高比例的T. brucei读数.
在对照血液或便样本中没有显著的T. brucei鉴定.
基因组组合从受感染的血液中获得了T. brucei contigs,准确度超过96%.

结论:

纳米孔测序表明了从元基因组样本分类和组装高变性基因组的潜力.
这项技术对研究像T. brucei.这样的宿主依赖寄生虫充满希望.
这项研究强调了纳米孔测序在具有挑战性的基因组研究场景中的实用性.

关键词:

长读序列的测序方式甲基基因组是指甲基因组中的一部分.纳米孔测序的测序原生动物原生动物在Trypanosome的研究中,我们发现了三子体.

更多相关视频

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...