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托西菲林对小鼠卵细胞成熟和质量的[in vitro]影响

Junjiao Wu¹, Jianbo Wu¹, Yanyan Xu¹

¹Department of Obstetrics-gynecology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

Iranian journal of basic medical sciences

|February 5, 2025

概括

托西菲林 (PTX) 改善了小鼠卵细胞的成熟和质量. 最佳的PTX度提高了核和细胞质成熟,同时减少了氧化应激,有利于生殖研究.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
细胞生物学细胞生物学

背景情况:

试管成熟 (IVM) 对于辅助生殖技术至关重要.
卵细胞质量是成功受精和胚胎发育的关键决定因素.
氧化应激会对卵细胞的成熟和质量产生负面影响.

研究的目的:

为了评估托西菲林 (PTX) 对小鼠卵细胞IVM的影响.
为了确定最佳的PTX度以改善卵细胞成熟和质量.

主要方法:

在IVM介质中培养小鼠卵细胞,具有不同的PTX度 (0-100μM).
评估核和细胞质成熟,包括生殖囊泡分解 (GVBD) 和第一个极体挤出 (PB1E).
评估的卵细胞质量标志物:皮质颗粒 (CG) 分布,螺旋结构,染色体对齐和活性氧物种 (ROS) 水平.

主要成果:

10,25和50μM的PTX显著改善了核成熟率.
10微米PTX显示了最佳的细胞质成熟,增强了CG分布,螺旋结构和染色体对齐.
10微米PTX治疗有效降低了细胞内ROS水平.

结论:

托西菲林补充剂可以提高小鼠卵细胞IVM和质量.
PTX通过优化CG分布,线完整性和染色体对齐来改善卵细胞成熟.
PTX减轻氧化应激,有助于IVM期间改善卵细胞质量.

关键词:

染色体是一种染色体.皮层颗粒是皮层中的颗粒.卵细胞成熟的过程潘托克西菲林 (pentoxifylline) 是一种药物.有反应性氧物种的反应性氧物种.螺旋的装置螺旋的装置

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