相关实验视频

Updated: May 29, 2025

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

研究E2F5表达在前列腺癌中循环的细胞外囊泡中的临床意义

Noushim Akram Huda¹, Souvik Chatterjee¹, Nahid Sultana²

¹Department of Urology, IPGMER and SSKM Hospital, Kolkata, West Bengal, India.

|February 5, 2025

概括

这项研究发现,E2F5在所有格里森等级的前列腺癌组织中高度过度表达. 血中的细胞外囊泡 (EV) 显示出作为预测前列腺癌进展的液体活检标记物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

前列腺特异性抗原 (PSA) 查可以改善前列腺癌的检测,但缺乏特异性,导致过度治疗瘤.
转录因子E2F5在前列腺癌 (PCa) 中被放大,并在细胞过程中发挥关键作用.
使用血液中的细胞外囊泡 (EVs) 进行液体活检,为生物标志物发现提供了一种最少的侵入性方法,解决PSA的局限性.

研究的目的:

通过将分子读数与组织病理学集成来完善前列腺癌诊断.
研究前列腺癌组织和EV中的E2F5表达.
评估EVs作为临床显著前列腺癌的生物标志物的潜力.

主要方法:

分析了100个前列腺活检组织中的E2F5表达 (4格里森等级和BPH) 使用免疫组织化学 (IHC).
从患者血中分离和特征细胞外囊泡 (EVs).
与格里森等级和EV特征相关的E2F5表达水平.

主要成果:

与良性前列腺增生 (BPH) 组织相比,所有格里森等级的E2F5显著上调,在格里森10表达最高,在格里森7表达最低 (3+4).
从血中分离的EV大小在30-200nm之间测量.
在E2F5过度表达和恶性前列腺组织之间显示出强烈的相关性.

结论:

在所有格里森等级的恶性前列腺活检组织中,E2F5高度过度表达,BPH组织作为对照.
从血中提取的细胞外囊泡 (EVs) 是一个潜在的液体活检标志物.
基于EV的生物标志物可以帮助预测疾病进展并改善前列腺癌患者的预后.

关键词:

E2F5 E2F5F5 E2F5 E2F5 E2F5 E2F5 E2F5 E2F5 电动汽车 (EV) 是一个在IHC中,IHC是指IHC.液体活检活检液体活检最少的侵入性侵袭性疾病分子读数的分子读数.

更多相关视频

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

Published on: February 3, 2023

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

Published on: May 15, 2014

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...