在约旦医院中从无生命表面分离出来的金黄色葡萄球菌的分子研究
O Al-Fawares1, R H F Bashabsheh1, I Y Natsheh2
1Al-Balqa Applied University, Faculty of Science, Department of Medical Laboratory Sciences, Al-salt, Jordan.
Brazilian journal of biology = Revista brasleira de biologia
|February 5, 2025
概括
约旦的医院表面显示出高污染率的黄金葡萄球菌和抗甲素黄金葡萄球菌 (MRSA). 加强感染控制和绝育对于对抗这些普遍的医院病原体至关重要.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 医院流行病学 医院流行病学
背景情况:
- 黄金葡萄球菌是医院感染的常见原因之一.
- 甲素耐药黄金葡萄球菌 (MRSA) 在医疗保健环境中构成重大威胁.
- 了解这些细菌在医院表面的流行程度对于感染控制至关重要.
研究的目的:
- 确定约旦四家主要医院在无生命表面上的金黄色葡萄球菌和MRSA的流行率.
- 为了确定特定的医院部门具有更高的污染率.
- 评估医院环境与细菌污染之间的相关性.
主要方法:
- 在各个医院部门抽取500个无生命的表面.
- 使用格拉姆染色和生物化学试验 (catalase,凝结酶,DNase,尿素酶,氧化酶,血解素) 鉴定细菌分离物.
- 在分子检测中检测Staphylococcus spp. (16S rRNA),S. aureus (可阿基因) 和MRSA (mecA基因) 通过PCR.
主要成果:
- 葡萄球菌 spp. 葡萄球菌 spp. 患病率为42.4% (212/500). 在美国,患病率为42.4% (212/500).
- 黄金菌的流行率为39.6% (198/500),由同基因检测到.
- 通过mecA基因检测到的MRSA患病率为16.2% (81/500).
- 在医院病房和细菌污染之间观察到显著的正相关性 (P < 0.01).
结论:
- 约旦的无生物医院表面有大量的黄金菌和MRSA.
- 特定的医院部门显示污染风险更高.
- 实施强有力的绝育和感染控制策略是必不可少的.
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