在低氧条件下,m6A修饰和转录量对植物压力颗粒中的mRNA组成的影响
Dawid Jakub Kubiak1, Michal Wojciech Szczesniak2, Karolina Ostrowska1
1Department of Cellular and Molecular Biology, Nicolaus Copernicus University, Lwowska 1, 87-100, Torun, Poland.
Journal of experimental botany
|February 5, 2025
概括
在应力颗粒 (SG) 组装中,N6-甲基氨酸 (m6A) 修饰的作用有限. 然而,细胞质中的m6A水平和转录量在应激反应期间调节mRNA分裂成SG.
科学领域:
- 分子生物学分子生物学
- 植物生物学 植物生物学
- 细胞生物学 细胞生物学
背景情况:
- 压力颗粒 (SGs) 是在细胞压力下形成的动态细胞质焦点.
- N6-甲基氨酸 (m6A) 是一个关键的表体转录组修饰调节植物mRNA.
- m6A在SG形成和组成中的作用在很大程度上是未知的.
研究的目的:
- 调查m6A对植物SG组装和组成的影响.
- 确定m6A水平,转录丰度和SG局部化之间的关系.
- 阐明mRNA分裂成SGs的调节机制.
主要方法:
- 在Lupinus angustifolius*中进行转录组分析.
- 在SG中对特定mRNA的局部化研究.
- 在不同成绩单中评估m6A水平.
- 对具有改变m6A水平的*Arabidopsis*突变物进行分析.
主要成果:
- 低氧反应基因转录 (ADH1,HUP7) 显示m6A水平低于家政基因.
- HUP7 mRNA,低m6A,局部到SG和细胞质,可能是由于高表达.
- 特别是在SG组装过程中观察到m6A的修改.
- 减少m6A的*Arabidopsis*突变体呈现出减少的多ARNA和较少的SGs.
结论:
- m6A修改对SG组件的直接影响有限.
- m6A水平和总体转录量之间的相互作用会影响mRNA分裂成SGs.
- 这些发现揭示了在压力下SG形成的新型监管机制.
更多相关视频
08:09Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica
Published on: September 20, 2024
395
11:04Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
Published on: November 30, 2015
13.2K
相关概念视频
01:53RNA Stability
33.2K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.2K
02:51mRNA Stability and Gene Expression
5.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.5K
02:41Responses to Drought and Flooding
10.6K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.6K
01:23Regulation of Expression at Multiple Steps
864
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
864
02:45Responses to Heat and Cold Stress
13.3K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.3K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K