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Updated: May 29, 2025

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使用 SEAM-Combi-OGAB 方法组合的非核糖体合成酶库

Varada Jagadeesh1, Nobuyuki Okahashi2,3, Fumio Matsuda2,3

  • 1Graduate School of Science, Technology, and Innovation, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.

ACS synthetic biology
|February 5, 2025
PubMed
概括

一种新的方法,SEAM-combi-Ordered Gene Assembly in Bacillus subtilis (SEAM-OGAB),可以有效地构建大型非核糖体合成酶 (NRPS) 基因库. 这种技术可以快速生成各种产品,用于各种应用.

关键词:
在此之前,NRPS是NRPS.这就是 SEAM-OGAB.在 SEAM-combi-OGABB 中使用.组合OGAB-OGAB是什么意思格拉米西丁S 是一种草药.皮帕斯塔提因与皮帕斯塔提因是不同的.

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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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