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维生素C可以缓解由Carassius auratus中的17α-甲基丸激素引起的肠损伤

Haiyan Zhao¹, Tongyao Li¹, Chenyang Li¹

¹College of Animal Science, Shanxi Agricultural University, Jinzhong, 030801, China.

Aquatic toxicology (Amsterdam, Netherlands)

|February 5, 2025

概括

维生素C (VC) 保护金鱼 (Carassius auratus) 免受由内分泌干扰化学物质17α-甲基 (MT) 引起的肠损伤. 25和50mg/kg的最佳VC剂量恢复了肠道健康和平衡的肠道微生物群.

科学领域:

环境毒理学环境毒理学
水生生物的健康水生生物的健康
营养生物化学营养生物化学

背景情况:

17α-甲基 (MT) 是一种在水生环境中普遍存在的内分泌干扰化学物质 (EDC).
MT对水生生物构成重大健康风险,特别是影响肠道完整性.
了解防范EDC引起的损害的保护策略对于水生生态系统的健康至关重要.

研究的目的:

为了研究维生素C (VC) 对Carassius auratus的MT诱导的肠损伤的保护作用.
为了确定VC的最佳剂量,以减轻MT的毒性.
阐明VC的保护作用背后的机制,包括对肠道屏障功能,炎症和肠道微生物群的影响.

主要方法:

在Carassius auratus被暴露在MT (50 ng/L) 和不同的VC度 (0,25,50,150 mg/kg) 中,持续了7,14,21天.
通过帕拉芬切片分析肠道组织,以检测病理变化.
评估了消化酶活性,基因表达 (肠道屏障和炎症标志物) 和肠道微生物群组成 (16S rDNA测序).

主要成果:

暴露于MT引起肠损伤,真空化,炎症 (TNF-α上调,IL-10下调),障碍功能受损 (Occludin,Muc2,Claudin-12下调) 和消化酶活性降低.
VC (25和50毫克/公斤) 显著改善了MT诱导的病理变化,恢复了小的长度,并使炎症和与屏障相关的基因的表达正常化.
MT破坏了肠道微生物群,增加了有害的Acinetobacter,而VC补充促进了有益的Lactobacillus和改善了消化酶活性.

结论:

暴露于MT严重损害了肠道屏障,并促进了Carassius auratus的炎症,部分是通过改变肠道微生物群.
维生素C,特别是25和50毫克/公斤,通过减轻MT诱导的肠损伤,显示出显著的保护作用.
维生素的好处归因于其恢复肠道屏障完整性的能力,减少炎症,并积极调节肠道微生物群体.

关键词:

17α-甲基测试的使用.卡拉西乌斯·奥拉图斯 (Carassius auratus) 是一个的动物.肠道屏障肠道屏障肠道屏障肠道菌群是什么意思肠道菌群维生素C 维生素C是一种

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