基于表面增强拉曼光谱 (SERS) 的尿液活检用于脏移植患者
Jiaqi Wang1, Fanxiang Meng1, Zhiyong Ma2
1State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, PR China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|February 5, 2025
概括
表面增强拉曼光谱 (SERS) 为监测移植患者提供了一种非侵入性方法. 这种先进的技术分析尿液以检测免疫排斥等并发症,改善患者护理.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 移植医学 移植医学
背景情况:
- 监测移植接受者免疫排斥和感染等并发症是当前技术的挑战.
- 现有的方法缺乏足够的灵敏度,特异性和持续动态监测能力.
研究的目的:
- 评估移植患者的生理状态,使用非侵入性表面增强拉曼光谱 (SERS) 进行液体活检.
- 探索用于早期检测移植并发症的新型SERS策略.
主要方法:
- 采用了两种SERS方法:一种使用体Ag组合膜基板的无标签策略和一种使用4 - 默卡波皮里丁 (MPY) 探针策略.
- 分析了脏移植患者在手术前和后的不同阶段的尿样.
- 利用机器学习算法来分析SERS光谱数据并构建预测模型.
主要成果:
- 在尿样中确定了与功能和免疫状态相关的关键生物标志物.
- 开发精确的机器学习模型,能够区分不同手术阶段的患者.
- 证明了SERS在敏感和特定检测移植后变化的潜力.
结论:
- 基于SERS的液体活检显示了对移植患者的非侵入性监测的前景.
- 这种方法可以帮助早期诊断免疫排斥,并提供有关患者康复的见解.
- 塞尔斯为移植管理提供了一个敏感,具体和动态的监测工具.
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