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CircHOMER1通过与HuR结合并稳定NOX4mRNA,促进二氧化诱导的肺纤维化

Qiuyun Wu¹, Qianyi Zhang², Chunmeng Jin²

¹School of Public Health, Xuzhou Medical University, Xuzhou 221004, China; Jiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou 221004, China.

Cellular signalling

|February 5, 2025

概括

循环RNA HOMER1 (circHOMER1) 通过通过HuR蛋白稳定NOX4mRNA,促进了性纤维化. 抑制cirCHOMER1可能为这种职业肺部疾病提供治疗策略.

科学领域:

职业医学职业医学是专业的.
分子生物学分子生物学
肺部医学肺部医学

背景情况:

病是一种严重的职业病,其特点是由于暴露于颗粒而导致的肺纤维化.
循环RNAs (circRNAs) 是纤维化过程中的新兴调节者,为化机制提供了潜在的见解.

研究的目的:

为了研究 circRNA HOMER1 (hsa_circ_0006916) 在二氧化引起的肺纤维化中的作用和机制.
探索circHOMER1作为症的潜在治疗标.

主要方法:

使用Actinomycin D,RNase R和核质分离试验评估 circHOMER1 的特征.
在暴露于二氧化颗粒的小鼠模型中利用了他的病理学和ELISA.
通过西部斑块和RT-qPCR检查了circHOMER1,HuR和NOX4的表达水平.
使用RNA免疫沉降 (RIP) 和共同传染试验分析了分子相互作用.

主要成果:

在纤维化纤维化中证实了cirCHOMER1的上调.
证明 circHOMER1 敲除抑制纤维细胞活力和纤维化标记物,减轻肺组织损伤.
揭示了circHOMER1直接与HuR结合,增强其蛋白质表达.
透露 circHOMER1 通过 HuR 稳定 NOX4 mRNA,增加 ROS 生产并促进纤维化纤维化.

结论:

环形RNA HOMER1 在二氧化诱导的肺纤维化中起着重要作用.
抑制cirCHOMER1为缓解病理学提供了潜在的治疗途径.

关键词:

这就是 HuRR 的意思.在NOX4中,NOX4是肺部纤维化肺部纤维化石是最重要的材料之一.在CircHOMER1中,环RNA 环RNA 是一个环RNA.

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