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来自Escherichia phage phi92的胆酸降解酶的表达和特征以及其水解物成分和结构的分析

Xiaotong Xu¹, Xiaoshuang Yan², Xuerong Jin³

¹Department of Food Science and Technology, Pukyong National University, Busan, Republic of Korea; Key Laboratory of Quantitative Synthetic Biology, Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

International journal of biological macromolecules

|February 5, 2025

概括

一种新型酶将细菌胆酸 (CA) 降解为较小的寡糖 (CAOS). 通过减少炎症和氧化应激,这些CAOS显示出在食品,化品和药品中使用的潜力.

科学领域:

微生物学和生物化学
酶学是一种酶学.
生物技术是生物技术.

背景情况:

结肠酸 (CA) 是一种细菌外聚糖,可以延长线虫的寿命,但面临着粘度挑战.
胆酸降解酶 (CAE) 可以克服这些粘度问题,从而实现实际应用.

研究的目的:

为了表达和描述来自Escherichia phage phi92.92的新型CAE.
使用新型CAE.进行CA寡糖 (CAOSs) 的产生和结构分析.
评估CAOSs的生物活动,重点关注抗炎和抗氧化特性.

主要方法:

优化CAE表达使用IPTG诱导在20°C24小时.
确定了CAE的最佳活性 (55°C,pH6.0) 和稳定性 (4-50°C,pH4.0-7.0).
使用GC-MS,LC-MS和NMR特征CAOS结构 (六糖,十二糖),识别β-1,4糖链接裂变.

主要成果:

通过裂解葡萄糖和糖之间的β-1,4糖化链,CAE有效地产生了CAOS.
在RAW 264.7巨细胞中,CAOSs减轻了H2O2诱导的氧化应激和炎症.
CAOSs抑制了氧化和甲的产生,增强了酶活性,并调节了亲和抗炎性基因表达.

结论:

来自Escherichia phage phi92的新型CAE有效地产生生物活性CAOSs.
CAOS显示出显著的抗炎和抗氧化潜力.
这项研究有助于开发CAOS用于食品,化品和制药行业的应用.

关键词:

酸降解酶 (CAE) 是一种可酸降解酶.埃舍里希亚菌体 phi92 phi92 橄糖化物橄糖化物

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