对CART在血清增强功能和焦虑症中的作用的新见解
Nagalakshmi Balasubramanian1,2, Ruixiang Wang1,2, Shafa Ismail1
1Department of Neuroscience and Pharmacology, University of Iowa, Iowa City, Iowa 52242.
概括
可卡因和胺调节转录 (CART) 抑制了背脊拉菲核 (DRN) 中的血清激素神经元,导致类似焦虑的行为. 这条来自埃丁格-韦斯特法尔核 (EWcp) 的CART通路显示了小鼠的性别特异性影响.
科学领域:
- 神经科学是一个神经科学.
- 神经内分泌学神经内分泌学
- 行为科学 行为科学
背景情况:
- 背脊核 (DRN) 中的血清 (5-HT) 系统调节与压力相关的行为.
- 可卡因和胺调节转录 (CART) 与这些行为有关.
- 在DRN中观察到CART轴突终端.
研究的目的:
- 研究CART和DRN5HTergic系统之间的相互作用.
- 确定CART在与焦虑相关的行为中的作用.
- 确定涉及的特定神经通路.
主要方法:
- 将CART的微输入到DRN中.
- 在体内用和5-HT生物传感器进行纤维光度测量.
- 在埃丁格-韦斯特法尔核 (EWcp) 中CART神经元的化学遗传激活.
主要成果:
- 在雄性小鼠中,DRN内CART输液诱导了焦虑效应.
- 卡特减少了5-HTDRN神经元中的c-Fos表达,并抑制了5-HT释放.
- CART EWcp→DRN预测的化学遗传激活在男性中模仿了这些效应,而不是女性.
- 卡特投射与非-5-HT神经元发生突触,抑制5-HT并激活GABAergic神经元.
结论:
- 卡特EWcp神经元调节5-HTDRN的神经传递.
- 通过对5-HT神经元的前抑制,CART促进类似焦虑的行为.
- 这种CART路径显示了焦虑调节中的性别特异性作用.
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