用可植入的微电极阵列微探测器对多巴胺进行电化学探测,其中包括在探测器上的氧化物参考电极
Dmitriy A Ruckodanov1, Nigel T Maidment2, Harold G Monbouquette1
1Chemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, United States.
ACS chemical neuroscience
|February 5, 2025
概括
一个可植入的微电极阵列与探头上的氧化物参考电极改善了多巴胺传感. 这种新的设计为研究像帕金森病这样的大脑疾病提供了高灵敏度和选择性.
科学领域:
- 神经科学是一个神经科学.
- 生物医学工程 生物医学工程
- 电化学 电化学 电化学
背景情况:
- 在体内监测多巴胺 (DA) 信号传递对于了解像帕金森病和药物滥用等脑部疾病至关重要.
- 外部参考电极 (例如,Ag/AgCl) 可能不稳定,并在体内诱导炎症反应.
- 将稳定的参考电极直接集成到微电极阵列 (MEA) 中是可靠的神经记录的理想选择.
研究的目的:
- 开发和评估一个可植入的微电极阵列 (MEA) 与一个集成的探头上氧化 (IrOx) 参考电极 (RE) 进行敏感和选择性的多巴胺 (DA) 检测.
- 为了比较DA传感的性能,使用在探头上的IrOx RE在两和三电极配置与传统的外部参考电极.
- 评估集成系统对常见的电活性干扰物的选择性,用于体内应用.
主要方法:
- 使用电化学沉积制造一个微探头MEA的制造,其中包括一个集成的探头上的IrOx RE.
- 测试集成的MEA在两个电极 (工作电极[WE]和RE) 和三个电极 (WE,RE和对应电极) 配置的DA传感.
- 量化DA传感灵敏度,检测极限 (LOD) 和对干扰物的选择性.
主要成果:
- 在集成和外部参考系统之间,可比的DA传感灵敏度 (∼2500 nA/(μM·cm2).
- 与外部引用相比,集成的三电极配置在DA检测方面实现了6倍低的LOD (∼9nM).
- 在集成的三电极设置中观察到基线噪声减少82%,这有助于改善LOD.
- 对常见的电活性干扰剂表现出极好的选择性 (1000:1).
结论:
- 在MEA中集成的探头上的IrOx RE显著提高了多巴胺传感性能,提供了超低的LOD和高选择性.
- 这种集成系统为体内神经科学研究的外部参考电极提供了稳定且不太炎症的替代方案.
- 开发的微探测器非常适合在神经和精神疾病的背景下对多巴胺信号的精确体内监测.
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