酒精中的新型甘油糖化物是由阿斯伯吉路斯里扎 (Aspergillus oryzae) 的α-葡萄糖酶A形成的
Yui Akiyama1, Kou Yoshizawa2, Asuka Yamada2
1Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
Journal of bioscience and bioengineering
|February 5, 2025
概括
萨克含有新型糖化物,包括多达四个与糖醇相关的葡萄糖单位,使用LC-MS和NMR识别. 一个主要的化合物,α-isomaltosylglycerol (α-iMG),在商业酒精中普遍存在.
科学领域:
- 食品化学 食品化学
- 生物化学 生物化学
- 酶学 是一种酶学.
背景情况:
- 萨克的生产涉及Aspergillus oryzae的α-葡萄糖酶,从而产生独特的葡萄糖类.
- 这些糖化物影响着 sake 的味道,并可能具有有益的生物活性.
研究的目的:
- 为了确定糖果中的新型转糖酶化产品.
- 描述这些新发现的化合物的结构和发病率.
主要方法:
- 液体染色学-质谱学 (LC-MS) 用于检测和识别糖化物.
- 在体外转糖酶化实验中使用重组阿尔法-葡萄糖酶A (AgdA).
- 核磁共振 (NMR) 光谱用于结构阐明.
主要成果:
- 在糖果中发现了二甲基烯酸甘油醇,三甲基烯酸甘油醇和四甲基烯酸甘油醇,这是第一次观察到如此长的葡萄糖链.
- 这些化合物在用AgdA破坏的Aspergillus oryzae造的酒中不存在.
- 识别alpha-isomaltosylglycerol (α-iMG) 作为一个主要的体外合成产品.
- 在商业糖果中检测α-iMG,平均度为1095 ppm.
结论:
- 已识别的葡萄糖是糖醇和葡萄糖单元的转糖化产物,由阿斯伯吉路斯或瑞泽的α-葡萄糖酶形成.
- 阿尔法-异盐糖醇 (α-iMG) 是商业酒精中常见的成分,标志着其首次在食品和饮料中出现.
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