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可持续的基于菌素的Ag纳米粒子合成和细胞毒性研究

Balázs Kakasi^1,2, Flóra Judit Varga¹, Márk Hopotószki¹

¹Air Chemistry Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, 10. Egyetem Str., Veszprém, 8200, Hungary.

Naunyn-Schmiedeberg's archives of pharmacology

|February 5, 2025

概括

银纳米颗粒 (Ag NPs) 合成了链杆菌素,在减少和稳定纳米颗粒方面发挥了双重作用. 这些抗生素稳定的AgNP通过破坏线粒体来证明对癌细胞的有效性,为纳米医学开发提供了潜力.

科学领域:

纳米技术纳米技术
生物医学工程生物医学工程
材料科学材料科学材料科学

背景情况:

抗生素耐药性是一个重大的全球健康挑战.
纳米粒子为生物医学应用提供与生物系统的动态相互作用.
用抗生素合成纳米颗粒为打击耐药性和增强治疗潜力的新方法提供了新的方法.

研究的目的:

合成和表征银纳米颗粒 (Ag NPs) 稳定通过抗生素 streptomycin.
评估这些AgNP对A549肺癌细胞的细胞毒性和线粒体影响.
探索抗生素稳定AgNP在纳米粒子合成中的双重作用及其在纳米医学中的潜力.

主要方法:

使用 estreptomycin 作为稳定剂合成 Ag NPs.
使用传输电子显微镜 (TEM),X射线衍射 (XRD) 和紫外线可见光谱 (UV-VIS) 进行Ag NP的表征.
通过流式细胞计和对A549肺癌细胞上的线粒体活性评估来评估细胞毒性.

主要成果:

形成了球形的Ag NP (10-20 nm),表现出没有聚合的稳定性.
在低度 (0.02毫米) 时,AgNP显示出低细胞毒性,LC50在1.7-1.9毫米之间.
在0.01mM Ag NP下观察到线粒体活性下降,这表明可能诱导细胞损伤.

结论:

结石素稳定的AgNP作为减少剂和稳定剂同时起作用,没有添加剂.
合成的纳米结构银颗粒证明了对测试的癌细胞系的有效性,这可能是由于线粒体损伤和自由基的产生.
这项研究强调了带有抗生素的纳米颗粒在开发先进的纳米药物,如药物输送和抗菌涂层等领域的潜力.

关键词:

抗生素抗生素是一种抗生素.生物启发的生物灵感细胞毒性细胞毒性线粒体活动活动银纳米颗粒银纳米粒子链杆菌素是什么?链杆菌素是什么?

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