无反应的线粒体膜潜在独立的光原体,具有活神经元成像的聚合诱导排放特征
Hojeong Park1, Guangle Niu2, Alex Y H Wong3
1Department of Chemistry, Institute for Advanced Study, State Key Laboratory of Neuroscience and Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Chemical communications (Cambridge, England)
|February 6, 2025
概括
使用聚合诱发发射 (AIE) 光原体创建了新的光稳定线粒体准探针. 这些探测器显示泄漏和细胞毒性减少,改善了线粒体成像应用.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 材料科学 材料科学 材料科学
背景情况:
- 线粒体是细胞能量生产和信号传递的关键器官.
- 准探头是研究线粒体功能和功能障碍的重要工具.
- 现有的探头往往会受到光漂白,泄漏和细胞毒性的影响,从而限制了它们的实用性.
研究的目的:
- 开发新的,光稳定的线粒体向探针,增强细胞保留.
- 为了研究聚合诱导发射 (AIE) 光源的结构-属性关系,用于线粒体成像.
- 在光稳定性,准效率和细胞毒性方面评估这些探针的性能.
主要方法:
- 合成新型AIE发光剂,其核心是基基,基链长度在基部分上有所变化.
- 光物理性质的表征,包括光量子产量和光稳定性.
- 使用光显微镜评估活细胞中的线粒体局部化和保留.
- 使用标准细胞活力试验评估探针细胞毒性.
主要成果:
- 开发的AIE探测器表现出极好的光稳定性和强烈的光发射.
- 在的部分上引入链显著增强了探头与线粒体膜的相互作用.
- 探测器证明了线粒体内的有效保留,即使在线粒体膜脱极化后.
- 与传统探针相比,新型探针显示细胞毒性显著降低.
结论:
- 基于AIE发光剂的新型光稳定型线粒体准探头成功开发.
- 设计的探头表现出增强的线粒体相互作用和保留,克服了现有探头的局限性.
- 这些AIE探针代表了先进的线粒体研究和诊断的有希望的工具.
相关概念视频
01:11Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
01:20Anatomy of the Eyeball
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...
01:22Photoreceptors and Visual Pathways
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...