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将基因组下一代测序和形态学结合起来,用于识别Coccidioides immitis:一个案例报告

Hong Liu¹, Kaixin Zhou¹, Chaoran Zhong¹

¹Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Frontiers in medicine

|February 6, 2025

概括

大基因组测序有助于诊断一种罕见的Coccidioides immitis脑膜炎病例. 将mNGS与传统方法相结合,为快速准确地识别这种真菌感染提供了一个至关重要的策略.

科学领域:

菌类学菌类学是指菌类学.
传染性疾病传染性疾病
临床微生物学临床微生物学

背景情况:

由Coccidioides spp.引起的Coccidioidomycosis是一种特定区域内在的全身性真菌感染.
中枢神经系统的参与,如脑膜炎,由于非特异性症状和负面的常规培养, presents诊断挑战.
型菌病在中国很少见,需要先进的诊断方法.

研究的目的:

报告一个使用元基因组下一代测序 (mNGS) 诊断的Coccidioides immitis脑膜炎病例.
突出结合mNGS与优化传统培养方法用于诊断罕见真菌感染的实用性.
强调开发病原体特异性诊断方法对菌菌病的重要性.

主要方法:

一名27岁的男性患者出现了暗示中枢神经系统感染的症状.
脑脊液 (CSF) 用常规的细菌培养和元基因组下一代测序 (mNGS) 进行了分析.
用优化的真菌培养条件来确认mNGS的发现,然后进行涂抹染色和形态分析.

主要成果:

常规CSF培养细菌的结果是阴性.
mNGS检测到CSF中的Coccidioides immitis的读数很低.
优化的真菌培养最终在12天后产生了积极的结果,证实了C. immitis.

结论:

在常规培养失败的脑膜炎病例中,mNGS可以检测出Coccidioides immitis.
将mNGS与增强的传统培养技术相结合,为诊断罕见的真菌感染提供了一个强大的策略.
这种综合诊断方法对于及时和准确的临床治疗Coccidioides immitis感染至关重要.

关键词:

虫虫 (Coccidioides immitis) 已经被发现.这种疾病是coccidioidomycosis.诊断策略诊断策略诊断策略脑膜炎是一种脑膜炎.基因组下一代测序 (mNGS) 是一种

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