Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

37.8K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
37.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hemisphere-wide evidence of Early Paleoindian megaherbivore specialization.

Science advances·2026
Same author

Genome Scanning Reveals the Genetic Basis of a Color Pattern Morphotype in an Island Population of the European Adder (Vipera berus).

Genome biology and evolution·2026
Same author

A comparison of ancient DNA yields across ossicles and the petrous bone reveals the best preservation in the stapes and incus.

Genome research·2026
Same author

Paleogenomes reveal the evolutionary relationship between modern and cave lions.

Cell·2026
Same author

Reply to Janssens and Mech: The possibility of natural wolf dispersal to Stora Karlsö.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

RpS12-mediated induction of the Xrp1<sup>short</sup> isoform links ribosomal protein mutations to cell competition.

Cell reports·2026
Same journal

From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.

Molecular ecology resources·2026
Same journal

EasyCen: A Lightweight Framework for Centromere Localisation and Repeat-Organisation Profiling in Telomere-to-Telomere Genomes.

Molecular ecology resources·2026
Same journal

A Practical Framework for GT-Seq Panel Optimization.

Molecular ecology resources·2026
Same journal

Comparison of Environmental DNA and Bulk DNA Metabarcoding for Assessing Terrestrial Arthropod Diversity Across Three Habitat Types on Guam.

Molecular ecology resources·2026
Same journal

pr2-Wormifier: A Bioinformatics Pipeline to Create Custom Reference Databases for Improved Metabarcoding of Marine Protists.

Molecular ecology resources·2026
Same journal

Individual Identification of Prey in Carnivore Scats.

Molecular ecology resources·2026
查看所有相关文章

相关实验视频

Updated: May 29, 2025

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

20.8K

一种高通量古代DNA提取方法,用于大规模样本查.

Alexandre Gilardet1,2, Edana Lord1,2, Gonzalo Oteo García1,3

  • 1Centre for Palaeogenetics, Stockholm, Sweden.

Molecular ecology resources
|February 6, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种具有成本效益,高通量古老DNA提取方法,使用96列板. 这种方法大大降低了用于选古生物学和考古学样本的成本和实验室时间.

关键词:
96个柱子的板块板块.提取DNA 提取DNA的方法古代的 DNA DNA 古代的 DNA DNA具有高通量功率的高通量功率.

更多相关视频

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.8K
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

3.7K

相关实验视频

Last Updated: May 29, 2025

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

20.8K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.8K
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

3.7K

科学领域:

  • 古代DNA研究 古代DNA研究
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 古代样品的大规模DNA查受到成本和吞吐量的限制.
  • 现有的用于DNA提取的机器人自动化是昂贵的,需要专门的人员.

研究的目的:

  • 开发一种高吞吐量,低成本的替代机器人古代DNA提取方法.
  • 优化DNA提取协议,以提高图书馆复杂性和基因组覆盖率.

主要方法:

  • 开发了一种新的基于96列板的DNA提取方法.
  • 新方法与常规单个MinElute列的比较.
  • 修改图书馆净化协议和化步骤 (Tween-20 补充).

主要成果:

  • 96列方法实现了与单列相似的内源DNA含量.
  • 产生了>0.1×覆盖面的线粒基因组,使得分类学赋值成为可能.
  • 优化的协议减少了碎片长度,DNA损伤和库复杂性的差异;成本降低了约39%.

结论:

  • 96列板法为高通量古代DNA查提供了一个可扩展和经济的解决方案.
  • 在化过程中添加Tween-20增强了库的复杂性和基因组覆盖范围.
  • 这种方法使得具有有限资源的实验室更容易获得古代DNA研究.