改进基于纳米孔测序的核心基因组MLST用于全球感染控制:一种针对诸如Burkholderia pseudomallei等GC丰富的病原体的策略

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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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Published on: February 23, 2021

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Sarah Weigl1, Johanna Dabernig-Heinz1, Fabian Granitz1

  • 1Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz, Graz, Austria.

Journal of clinical microbiology
|February 6, 2025
PubMed
概括

牛津纳米孔长读测序 (ONT-LR) 为细菌基因组监测提供了一个具有成本效益的替代方案. 一个优化的ONT-LR工作流显著减少了核心基因组多部位序列类型 (cgMLST) 中的错误,用于诸如Burkholderia pseudomallei.等富含GC的病原体.

关键词:
伯克霍尔德里亚是假名的.通过DNA甲基化.纳米孔测序的测序基于WGS的细菌类型识别.核心基因组 MLST 的核心基因组长阅读序列的测序.质性化症是什么

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