在考虑生物工程方面,诱导多能干细胞的扩展:第一部分
Samuel Lukas Schneider1, Misha Alexander Teale1, Stefan Seidel1
1Centre for Cell Cultivation Techniques, Tissue Engineering, and Medical Biology, Institute of Chemistry and Biotechnology, ZHAW Zurich University of Applied Sciences, Grüentalstrasse 14, 8820, Wädenswil, Switzerland.
Applied microbiology and biotechnology
|February 6, 2025
概括
用于治疗的人类诱导多能干细胞 (hiPSCs) 的可扩展扩张是使用生物反应器中的微载体实现的. 这种方法确保了细胞活力和分化潜力,为临床应用铺平了道路.
科学领域:
- 干细胞生物学 干细胞生物学
- 生物技术是生物技术.
- 生物工程是生物工程.
背景情况:
- 人类诱导多能干细胞 (hiPSCs) 的高效和可扩展扩张对于它们的治疗应用至关重要.
- 混合生物反应器中的微载体 (MC) 为附着细胞扩张提供了一个有前途的解决方案.
研究的目的:
- 开发和扩大使用MCs在水箱生物反应器中用于hiPSCs的准 perfusion 扩张程序.
- 为了证明这种方法对大规模hiPSC种植的可行性和有效性.
主要方法:
- 工艺开发涉及优化旋转瓶中的媒介交换和MC涂层 (Synthemax II).
- 扩展到1.3L的生物反应器使用Zwietering的悬浮标准 () 来实现最佳的细胞分布.
- 计算流体动力学和生物工程分析为了解过程理解和优化提供了信息.
主要成果:
- 一个hiPSC准 perfusion 扩展程序成功建立和扩展.
- 获得了大约26的膨胀系数,在5天内产生了超过3 × 10^9个细胞.
- 在整个种植过程中,hiPSC保持了生存能力,身份和差异化潜力.
结论:
- 开发的基于MC的准 perfusion 系统对于可扩展的hiPSC扩展是有效的.
- 均的细胞分布和附着于MCs对于高效的培养至关重要.
- perfusion 策略支持 hiPSC 培养,并有利于干细胞疗法.
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