Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Effects of biochar derived from different feedstocks on soil microbial nutrient limitation in a <i>Phyllostachys edulis</i> forest.

Ying yong sheng tai xue bao = The journal of applied ecology·2026
Same author

Altered heart rate variability is associated with all-cause mortality in patients with co-morbid insomnia and obstructive sleep apnea.

Sleep·2026
Same author

Dual mode analysis of lead ions in regulation of intestinal flora <i>via</i> split-DNA re-assembly catalyzed colorimetric reaction and <i>trans</i>-cleavage activity of Cas12a/crRNA.

Analytical methods : advancing methods and applications·2026
Same author

Clinic-based characteristics of animal-related injury presentations in Hangzhou, China: a 10-year retrospective analysis.

Frontiers in public health·2026
Same author

Development of a vascularized multi-organoid-on-a-chip to model the heart-islet axis in diabetic cardiomyopathy.

Bioactive materials·2026
Same author

The role of jasmonates in plant root growth and development.

Frontiers in plant science·2026
Same journal

Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model.

STAR protocols·2026
Same journal

Overcoming the challenges of genome-editing essential genes.

STAR protocols·2026
Same journal

Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice.

STAR protocols·2026
Same journal

Protocol for identifying functional regulatory mutation blocks by integrating genome sequencing and transcriptome data.

STAR protocols·2026
Same journal

Protocol for in vitro phase separation of ARID3B proteins.

STAR protocols·2026
Same journal

Protocol for isolating human extracellular vesicles from nephrotic urine and enriching autoimmunoglobulin-triggered extracellular vesicles.

STAR protocols·2026
查看所有相关文章

相关实验视频

Updated: May 29, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

使用最佳基于传输的基因过算法,对空间分辨率的转录组学数据进行无效化的协议.

Lin Du1, Jingmin Kang2, Jie Li3

  • 1College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Beijing 102601, China.

STAR protocols
|February 6, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了SpotGF,一种新的基因过方法,用于减少空间解析转录组学 (SRT) 数据中的噪声. 该协议提高了SRT数据质量和下游分析性能,而不改变原始基因表达.

关键词:
生物信息学是一种生物信息学.计算机科学 计算机科学健康科学 卫生科学 卫生科学系统生物学 系统生物学

更多相关视频

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

2.9K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.1K

相关实验视频

Last Updated: May 29, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K
Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

2.9K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.1K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 空间解析的转录组学 (SRT) 数据容易受到组织固定和细胞溶解等实验程序的噪声的影响.
  • 在样本处理期间的转录扩散引入了文物,使精确的空间基因表达分析复杂化.

研究的目的:

  • 介绍一项使用最佳运输基因过算法称为SpotGF的SRT数据拒绝的协议.
  • 提高SRT数据的质量和可靠性,以加强下游生物解释.

主要方法:

  • 该协议详细说明了SRT数据集的数据准备步骤.
  • 它涉及基于最佳传输原则计算基因过的SpotGF得分.
  • 描述了确定过门和生成无证数据的方法.

主要成果:

  • 该SpotGF算法通过过杂的基因表达信号,有效地拒绝SRT数据.
  • 消毒过程不会改变原始的原始基因表达值.
  • 该协议可以提高SRT数据质量.

结论:

  • 使用SpotGF提供的协议为拒绝SRT数据提供了一个有效的策略.
  • 改进的SRT数据质量有助于更准确,更强大的下游分析.
  • 这种方法为研究人员提供了一个有价值的工具,研究空间转录学.