对检测器噪声和灵敏度对血清影响的比较研究SERS分析:非传染性疾病歧视的例子
Lyudmila A Bratchenko1, Yulia A Khristoforova1, Irina A Pimenova1
1Department of Laser and Biotechnical Systems, Samara University, Samara, Russia.
Journal of biophotonics
|February 6, 2025
概括
表面增强的拉曼光谱 (SERS) 能够有效地区分慢性心力衰竭和慢性阻塞性肺病,使用低成本和高分辨率的探测器. 在这项研究中,检测器质量对疾病分类准确性的影响很小.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 分析化学 分析化学
- 医学诊断 医学诊断 医学诊断
背景情况:
- 表面增强拉曼光谱法 (SERS) 是一种用于分子分析的强大技术.
- 血清分析为疾病诊断提供了一种非侵入性的方法.
- 区分慢性心力衰竭 (CHF) 和慢性阻塞性肺病 (COPD) 是临床上有意义的.
研究的目的:
- 为了比较使用非冷却探测器 (EnSpectr R785) 与高光谱分辨率探测器 (Renishaw) 的SERS血清分析的诊断性能.
- 评估探测器质量的影响,对对CHF和COPD患者的分类准确性的影响.
主要方法:
- 使用SERS分析了CHF和COPD患者的血清样本.
- 采用了两种探测系统:一种低成本的非冷却探测器 (EnSpectr R785) 和一种高分辨率探测器 (Renishaw).
- 根据两种设置的SERS光谱数据评估了分类准确性.
主要成果:
- 在SERS分析中,EnSpectr (0.81) 和Renishaw (0.84) 设置之间的疾病分类准确度相似.
- 检测器质量和分类准确性之间的关系被发现是微不足道的.
- 与EnSpectr (721, 1051, 1665 厘米-1) 相比,Renishaw 设置发现了更多重要的光谱波段 (611, 675, 720, 804, 1187, 1495, 1847 厘米-1).
结论:
- 低成本和高分辨率的SERS检测器都能有效地区分CHF和COPD患者.
- 虽然分类准确度相似,但高分辨率探测器为识别特定疾病相关的光谱标记提供了更大的潜力.
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