释放植物细胞生产有价值蛋白质的全部潜力:挑战和创新的战略
Jianfeng Xu1, Paula PerezSanchez2, Shekoofeh Sadravi3
1Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USA; College of Agriculture, Arkansas State University, Jonesboro, AR 72401, USA.
Biotechnology advances
|February 6, 2025
概括
植物细胞生物生产提供可持续的蛋白质生成,但面临着挑战. 建议采用细胞工程策略,包括新的蛋白质合作伙伴和细胞壁修饰,以提高生产力和克服局限性.
科学领域:
- 生物技术是生物技术.
- 植物科学 植物科学
- 生物加工是一种生物加工.
背景情况:
- 基于植物细胞的系统在可持续生物生产方面比哺乳动物细胞培养和整个植物具有优势.
- 阻碍植物细胞生物生产的关键挑战包括生产率低,蛋白质效率改变,细胞凝聚,遗传不稳定性和冷保存困难.
研究的目的:
- 为了应对植物细胞生物生产的关键挑战.
- 提出创新的蜂工程策略,以释放平台的全部生产潜力.
主要方法:
- 设计新的蛋白质合作伙伴,以提高积累和功能.
- 利用精确的基因整合来改善转基因转录.
- 实施精英细胞系的先进查和维护方法.
- 利用基因组编辑进行细胞工程和优化.
- 专注于细胞壁工程 (纤维素/pectin 缺乏) 进行形态修饰.
主要成果:
- 拟议的战略旨在显著提高重组蛋白质的积累和功能.
- 预计先进的基因整合和细胞系维护方法将提高生产率和稳定性.
- 细胞壁工程为修改生物生产的植物细胞形态提供了新的方法.
结论:
- 创新的细胞工程对于克服当前植物细胞生物生产的局限性至关重要.
- 未来的研究应该专注于实施这些策略,以实现基于植物细胞的制造业的全部潜力.
- 本综述提供了前性观点,以激发该领域的进步.
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