大黄鱼 (Larimichthys crocea) 类似于 VII 凝血因子的分子特征及其对巨细胞增殖和两极分化的作用
Yueming Chen1, Han Zhao1, Shuangshuang Cao1
1State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Fish & shellfish immunology
|February 6, 2025
概括
这项研究在大型黄色鱼中发现了一种类似于凝血因子VII的基因,证明了它的免疫作用. 该因子表现出抗菌性质,并影响巨细胞的免疫反应,增强宿主防御机制.
科学领域:
- * 免疫学 免疫学
- * 鱼类生物学 鱼类生物学
- * 凝血系统凝血系统
背景情况:
- * 凝血系统对于伤口愈合和免疫防御至关重要.
- * 凝血因子VII (FVII) 启动了外部通路,但其免疫功能,尤其是鱼类,尚未得到充分研究.
- * 大黄 (Larimichthys crocea) 是一种重要的经济鱼类.
研究的目的:
- * 克隆和鉴定大型黄的FVII类基因 (LcFVIIL).
- * 调查LcFVIIL的免疫功能,包括它对抗菌活性和巨细胞行为的影响.
- *阐明凝血系统在鱼类天生的免疫力中的作用.
主要方法:
- *对LcFVIIL基因进行克隆和序列分析.
- *在不同组织和细菌挑战下对基因表达的分析.
- *生产重组LcFVIIL轻链 (rLcFVIIL-LC) 并评估其生物活动.
- *巨细胞增殖测定,细胞因子表达分析 (IL-1β,IL-6,TGF-β) 和反应性氧物种 (ROS) 活性测量.
主要成果:
- *成功克隆了LcFVIIL基因,揭示了脊椎动物FVIIs典型的保存域.
- *LcFVIIL在肝脏和大脑中表达高,并且在暴露于Pseudomonas plecoglossicida时在巨细胞中升级.
- *重组LcFVIIL光链显示显著的抗菌活性对抗阳性细菌,促进了巨细胞的增殖,诱导了促炎细胞因子 (IL-1β,IL-6),抑制了抗炎细胞因子 (TGF-β),并增加了ROS活性,表明M1巨细胞的两极分化.
结论:
- * LcFVIIL基因在大黄的先天免疫反应中起作用.
- *LcFVIIL具有抗菌性质,并调节巨细胞功能,有助于宿主防御.
- *这些发现扩大了对鱼类免疫力中的凝血因子功能的理解.
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