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基于聚合诱导的发射的体光探针用于检测Ni2+和Zn2+在不同的缓冲系统中

Dajiang Wang¹, Ping Ji^1,2, Xiaomei Liu¹

¹Department of Pharmacy, West China Hospital, Sichuan University, Chengdu, China.

Luminescence : the journal of biological and chemical luminescence

|February 6, 2025

概括

一种新的化发光探针TPE-HN,为检测重金属 (Ni2+) 和 (Zn2+) 提供了一种快速而灵敏的方法. 该探头适用于活细胞成像,解决与这些污染物相关的环境和健康问题.

科学领域:

环境科学环境科学
分析化学分析化学
生物技术是生物技术.

背景情况:

重金属污染,特别是 (Ni2+) 和 (Zn2+),对全球环境和人类健康构成重大风险.
灵敏和快速的分析方法对于准确检测Ni2+和Zn2+至关重要,以减轻污染和健康危害.

研究的目的:

设计和合成一种新的化探针,用于灵敏和快速检测Ni2+和Zn2+.
为了利用聚合诱导排放 (AIE) 效应进行适合生物应用的"启动"测试.

主要方法:

合成一个化发光探针TPE-HN,将一个序列与四乙烯 (TPE) 连接起来.
基于AIE效应的快速"启动"试验的开发,用于检测Ni2+和Zn2+.
评估不同缓冲系统中的探针性能,并评估其细胞透性和生物毒性.

主要成果:

在各种缓冲系统中,TPE-HN探测器成功地检测到了Ni2+和Zn2+的质量.
在15%的缓冲中达到Ni²⁺/Zn²⁺的9.613μM和在20%的缓冲中达到Ni²⁺的1.215μM的检测极限.
证明了高灵敏度,优异的细胞透性和低生物毒性,使生物环境中的Ni2+和Zn2+能够在活细胞中成像.

结论:

该TPE-HN光探针提供了一种敏感,快速和细胞透的方法来检测Ni2+和Zn2+.
该探测器适用于活细胞成像,这突显了它在监测生物系统中的重金属污染方面的潜力.
这项研究有助于开发用于环境监测和重金属毒理研究的先进工具.

关键词:

在Ni2+中.Zn2+ Zn2+ Zn2+ Zn2+ Zn2+ Zn2 聚合引发的排放量聚合引发的排放量细胞图像细胞图像体光探针的探测器

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