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一种简单的快速HPLC-UV方法,用于评估Darbepoetin Alfa中的C端氨酸含量

Dmitry I Zybin1, Anatoly A Klishin1, Natalia V Orlova1

  • 1PHARMAPARK LLC, Moscow, Russia.

Biomedical chromatography : BMC
|February 7, 2025
PubMed
概括

一种新的HPLC-UV方法快速评估了 darbepoetin alfa生物类似剂中的C端Arg166含量. 这种质量控制工具有助于开发和优化重组药物生产.

关键词:
在C端的阿尔金因中.在HPLC中使用HPLC.达尔贝波埃丁阿尔法 (Darbepoetin alfa) 是一种

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Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...