肺癌的元基因组下一代测序低呼吸道感染的诊断和特征微生物组的特征
Yao Liu1, Bohan Yang1, Qi Qi1
1Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Frontiers in cellular and infection microbiology
|February 7, 2025
概括
与传统方法相比,甲基因组下一代测序 (mNGS) 在肺癌患者的下呼吸道感染 (LRTI) 中提供了优越的病原体检测. 它还描述了呼吸道微生物组及其与肺癌发生的联系.
科学领域:
- 在瘤学瘤学.
- 微生物学 微生物学
- 传染性疾病 传染性疾病
背景情况:
- 下呼吸道感染 (LRTI) 在肺癌患者中构成诊断挑战.
- 基因组下一代测序 (mNGS) 在诊断LRTIs和特征呼吸道微生物群中的作用需要进一步调查.
研究的目的:
- 评估mNGS在肺癌患者中对LRTIs的诊断性能.
- 使用mNGS在肺癌患者下呼吸道 (LRT) 微生物群的特征.
- 探索与肺癌发生相关的潜在微生物功能.
主要方法:
- mNGS用于诊断LRTIs,并分析肺癌患者的LRT微生物组.
- 基因组变异分析 (GSVA) 用于评估已识别的微生物基因的功能潜力.
- 诊断性能与基于培养的方法和病毒DNA量化进行了比较.
主要成果:
- 与唾液培养 (34.5%) 相比,mNGS在肺癌患者的LRTI中显示出明显更高的病原体检测率 (83.3%).
- 多微生物感染比单微生物感染更常见.
- mNGS确定了Prevotella,Streptococcus,Veillonella,Rothia和Capnocytophaga作为普遍存在的属,GSVA揭示了与瘤代谢和信号通路的联系 (PI3K,Hippo,p53).
结论:
- mNGS显著优于基于培养的方法来诊断肺癌患者的LRTIs.
- mNGS提供了对LRT微生物组组成及其对肺癌发生的功能影响的宝贵见解.
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