热 (Capsicum annuum) 中SR基因的剪接和表达动态:调节多样性和应激状态下的保护
Lin Li1, Yueqin Zhang1, Rui Zhang1,2
1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.
Frontiers in plant science
|February 7, 2025
概括
研究人员在中发现了23种富含血清素/素 (SR) 的蛋白质,揭示了它们在应激耐受性和适应性方面的作用. 这些CaSR基因中的替代拼接 (AS) 增强了植物的弹性,为开发抗压作物提供了洞察力.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物化学 生化学
背景情况:
- 富含氨酸/氨酸 (SR) 的蛋白质是植物基因表达的关键调节者.
- 了解热 (Capsicum annuum) 等作物中的SR蛋白功能对于改善耐压力至关重要.
研究的目的:
- 为了识别和表征中的SR蛋白质.
- 研究SR蛋白和替代拼接 (AS) 在对环境压力的反应中的作用.
- 探索植物物种中SR蛋白调节的进化保护.
主要方法:
- 23个CaSR基因的基因鉴定和表征.
- 对CaSR蛋白质的遗传学分析.
- 促进者地区分析.
- 在盐,干旱和寒冷压力下进行基因表达分析.
- 跨不同组织的替代拼接 (AS) 分析.
- 跨物种的比较分析与阿拉比多普西斯和西红.
主要成果:
- 在中确定了23个CaSR基因,分为七个子家族.
- SR蛋白与其他植物的同类蛋白具有很高的相似性.
- 推动者区域包含压力,光线,激素和与生长相关的元素.
- 盐,干旱和寒冷压力对CaSR基因表达进行了上调.
- 观察到替代拼接,主要是内部保留,并因组织而异.
- AS事件经常影响RNA识别动机 (RRM) 域,表明受保护的调节.
结论:
- CaSR蛋白质在的耐压力方面发挥着重要作用.
- 替代拼接是提高热中的植物适应能力的关键机制.
- 在植物中,SR蛋白调节,特别是影响RRM域的AS,在进化过程中是保留的.
- 研究结果为开发抗压作物品种提供了洞察力.
相关概念视频
02:18Alternative RNA Splicing
20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
01:32RNA Splicing
55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K
02:45Responses to Heat and Cold Stress
13.3K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.3K
01:36What is Gene Expression?
8.4K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.4K
01:23Regulation of Expression at Multiple Steps
864
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
864
02:51mRNA Stability and Gene Expression
5.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.5K