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热带菌改变了肠道上皮细胞中的屏障透性和Claudin-1组织

Ha The Doan¹, Yi-Ling Chiu¹, Li-Chieh Cheng¹

¹Department of Life Science, National Taiwan University, Taipei, Taiwan.

Journal of physiological investigation

|February 7, 2025

概括

热带菌 (Candida tropicalis) 是一种非白菌,在炎症性肠病 (IBD) 模型中损害了肠道屏障功能. 这项研究揭示了它对上皮膜透性和紧结蛋白的特定影响,为IBD病原体提供了洞察力.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

炎症性肠病 (IBD) 涉及慢性肠道炎症和屏障功能障碍,通常与肠道微生物群有关. 虽然Candida albicans涉及,但Candida tropicalis等非白虫Candida物种在IBD中的作用不太清楚.
高水平的Candida tropicalis与IBD的发展相关,但其对肠道屏障完整性的具体影响尚不清楚.

研究的目的:

用Caco-2细胞模型研究Candida tropicalis对肠道屏障功能的影响.
阐明C. tropicalis通过哪些机制影响肠道上皮细胞和屏障完整性.

主要方法:

利用极化Caco-2细胞单层来模拟肠道上皮.
评估了体电阻 (TEER) 和对小分子 (lucifer黄色) 和大分子 (FITC-dextran) 透性的变化.
检查了紧接口蛋白质claudin-1.1的内部化.
将C.tropicalis的效果与齐莫桑 (dectin-1联结体) 治疗进行了比较.

主要成果:

热带菌明显改变了TEER,增加了黄的透性,但不是FITC-dextran.
在暴露于C. tropicalis.后,在Caco-2细胞中观察到紧结蛋白claudin-1的内部化.
齐莫桑治疗诱导了克劳丁-1的再分配,但没有影响单层透性,这表明C. tropicalis.具有特定的作用.

结论:

热带菌 (Candida tropicalis) 特别影响肠道屏障的完整性和肠道上皮细胞中小溶液的透性.
这些发现突出了C. tropicalis作为潜在的IBD病原体的贡献者,通过破坏肠道屏障.

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