对海藻Chlorella sp 进行交互性毒性作用,其中包括美特罗尼达,狄克洛芬,伊布洛芬和功能不同的纳米塑料Chlorella
Camil Rex M1, Bikram Poddar1, Sanmitra Mandal1
1Centre for Nanobiotechnology, VIT, Vellore, Tamil Nadu, India. amitav@vit.ac.in.
Environmental science. Processes & impacts
|February 7, 2025
概括
制药产品 (PPs) 和纳米塑料 (NPs) 具有敌对的相互作用,减少了对海洋微藻的整体毒性. PPs通过阻碍NP-藻类相互作用,减少氧化应激和增强光合作用来降低NP毒性.
科学领域:
- 环境毒理学环境毒理学
- 海洋生物学 海洋生物学
- 纳米技术纳米技术
背景情况:
- 制药产品 (PP) 和纳米塑料 (NP) 是海洋生态系统中新出现的污染物.
- 聚乙烯纳米塑料 (PSNPs) 具有氨基 (NH2-PSNPs) 和碳素 (COOH-PSNPs) 功能,对海洋生物构成风险.
- 对于PPs和PSNPs对海洋微藻的联合影响尚不清楚.
研究的目的:
- 在海洋微藻Chlorella variabilis上研究PPs (美트로尼达,迪克洛菲纳克,易布洛芬) 和PSNP的单独和联合毒性.
- 评估 PPs 对 PSNP 毒性的影响,并阐明底层机制.
- 评估暴露时藻类生理和生化参数的变化.
主要方法:
- 毒性评估包括生长抑制,反应性氧物种 (ROS) 生产,甲酸 (MDA) 含量,抗氧化活性和光合作用活性.
- 暴露后检查了藻类的表面功能组.
- 皮尔森相关性和集群热图分析被用于毒性终点相关性.
- 测量了泽塔潜力和水力动力学尺寸,评估了NP的稳定性.
主要成果:
- 在PPs的存在下,PSNP变得不稳定,导致聚合并减少与藻类细胞的相互作用.
- 纯净的PPs显示出最小的毒性,而纯净的PSNP则导致显著的生长抑制.
- 对PP和PSNP的联合暴露导致毒性低于原始PSNP,表明存在对抗性相互作用.
- PPs通过减少氧化应激和增强光合作用活性来减轻PSNP毒性.
结论:
- 制药产品可以调节纳米塑料对海洋微藻的毒性.
- PP和PSNP之间的对抗性相互作用与NP的物理不稳定性和改善藻类生理反应有关.
- 这些发现凸显了海洋环境中新出现的污染物的复杂相互作用.
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