蛋白质预涂层以度依赖的方式突破了肠粘膜中转移素功能化纳米粒子的生物宏分子屏障
Dan Yang1,2, Yao Zhou1,2, Mengqi Wang1,2
1School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi 710021, China.
Journal of agricultural and food chemistry
|February 7, 2025
概括
研究人员开发了一种使用牛血清白蛋白 (BSA) 预涂层转移素修饰纳米颗粒 (Tf-NP) 的新方法. 这种"活性蛋白冠状" (PC) 策略增强了细胞吸收和肠道透性,用于口服药物输送.
科学领域:
- 纳米技术纳米技术
- 生物材料科学 生物材料科学
- 药理学 药理学是指药理学的学科.
背景情况:
- 在生理环境中,生物大分子在纳米粒子 (NP) 上形成蛋白质冠冕 (PC).
- 电脑显著影响纳米粒子的行为,特别是在积极向的NP,如转移素修饰的NP (Tf-NP).
- 被动PC的抑制作用阻碍了Tf-NP在生物系统中的效率.
研究的目的:
- 设计一个设计一个.
- 活性蛋白冠状病毒冠状病毒.
- (APC) 来抵消被动PC对Tf-NP的抑制作用.
- 为了提高积极向纳米颗粒的口服管理效率.
主要方法:
- 用牛血清白蛋白 (BSA) 进行预涂层的Tf-NP的制造,形成B@Tf-NP,形成APC.
- 关于粒子大小,分散和物理稳定性的B@Tf-NP的表征.
- 评价细胞吸收在肠细胞和小鼠的肠道透性.
主要成果:
- B@Tf-NP与Tf-NP具有可比的粒子大小,分散和物理稳定性.
- B@Tf-NP显著增强了肠细胞中的细胞吸收.
- 在小鼠中,B@Tf-NP证明了肠道透性的改善.
- 确定了适当的BSA与Tf度比,用于及时暴露Tf.
结论:
- 将Tf-NP与BSA进行预涂层,可以创建一个克服被动PCs抑制作用的APC.
- 该APC策略增强纳米粒子的细胞吸收和肠道透性,用于口服.
- 这种方法提供了一个简单而有效的平台,用于设计积极向的NP,以克服口服管理的障碍.
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