聚硫化物在无氧条件下生长的微生物中促进蛋白质二硫化键的形成
Yuping Xin1, Qingda Wang1, Jianming Yang2
1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Applied and environmental microbiology
|February 7, 2025
概括
多硫化物在无氧条件下促进微生物中蛋白质二硫化键 (DSB) 的形成. 这一发现通过支持必要的蛋白质结构和功能,提高了在氧气有限的环境中微生物的生存率.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 环境科学 环境科学
背景情况:
- 多硫化物在无氧环境中对于硫循环和氧化还原转换至关重要.
- 在无氧条件下微生物的生存依赖于蛋白质二硫化键 (DSB) 的形成.
- 聚硫化物与无氧DSB形成之间的联系以前没有研究过.
研究的目的:
- 在无氧条件下调查聚硫化物在调解蛋白质DSB形成中的作用.
- 确定聚硫化物是否可以在无氧环境中增强微生物的生存和生长.
主要方法:
- 在无氧条件下用聚硫化物处理纯化蛋白质 (roGFP2,Trx1,DsbA).
- 在经过处理的蛋白质和整个微生物细胞 (大肠杆菌,schizosaccharomyces pombe,cupriavidus pinatubonensis) 中分析DSB的形成.
- 在转基因大肠杆菌菌株 (ΔdsbB, ΔdsbA) 中进行多硫化处理后,对微生物生长和细胞内DSB水平的评估.
主要成果:
- 聚硫化物在roGFP2,Trx1和DSbA蛋白质中诱导了分子内DSB的形成 *in vitro*.
- 聚硫化物治疗恢复了无氧 *E. coli* ΔdsbB突变体的生长,并增加了细胞内DSB水平.
- 聚硫化物促进了DSB的形成和恢复了无氧大肠杆菌* ΔdsbA突变体的生长,并增加了S. pombe* 和C. pinatubonensis* 的DSB含量.
结论:
- 聚硫化物有效地调解蛋白质DSB的形成,独立于氧气和已知的酶系统.
- 这种聚硫化物介导的DSB形成有利于微生物在无氧息地中的生存和适应.
- 研究结果揭示了一种新的机制,通过增强蛋白质稳定性,将聚硫化物与微生物无氧适应联系起来.
关键词:
该系统的DSB系统.无氧生长的无氧生长.聚硫化物多硫化物蛋白质二硫化物键是一种蛋白质二硫化物键.s-Glutathionylation是什么意思?s-glutathionylation是什么意思?s-glutathionylation是什么意思?s-glutathionylation是什么意思?更多相关视频
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