相关实验视频

Updated: May 29, 2025

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

在血和血清中基于MS的蛋白质组的多中心纵向质量评估

Oliver Kardell¹, Thomas Gronauer¹, Christine von Toerne¹

¹Metabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich 80939, Germany.

Journal of proteome research

|February 7, 2025

概括

针对临床蛋白质组学的标准化质谱 (MS) 工作流程显示出有前途. 一项多实验室研究证实了在血清和血样本中检测出71种蛋白质的可重复性检测,其中包括22种FDA批准的生物标志物.

科学领域:

临床蛋白质组学临床蛋白质组学
基于质谱 (MS) 的工作流程
生物分析方法的开发方法

背景情况:

为临床应用推进基于质谱 (MS) 的蛋白质组学,需要为临床样本提供标准化的工作流程.
关键的生物分析参数,如灵敏度,准确度和精度需要优化,以获得可靠的临床测试.
血清和血是临床诊断的关键生物流体.

研究的目的:

用临床相关样本 (血清和血) 评估基于MS的蛋白质组学工作流程的性能.
评估不同LC-MS/MS设置的灵敏度,精度和可重复性.
在临床环境中建立基于MS的测量基线.

主要方法:

这是一项纵向的循环研究,涉及来自MSCoreSys倡议的八个蛋白质组学实验室.
使用来自主要供应商 (ThermoFisher,Bruker,Evosep,Waters) 的多种LC-MS/MS系统.
在血清和血中监测蛋白质的识别,定量,灵敏度,精度和可重复性.

主要成果:

在各种MS仪器中,蛋白质识别范围从300到400个ID不等.
在所有血清和血设置中都可复制地检测到71种蛋白质.
22个FDA批准的生物标志物使用无标签量化进行了可重复量化 (CV <20%).

结论:

该研究表明,在临床环境中基于MS的血清和血分析具有前景的基线.
标准化工作流程提高了蛋白质组学测量的可复制性和可靠性.
这项工作支持基于MS的蛋白质组学向常规临床实用性的进步.

关键词:

这是LC-MS/MS.在R包中,mpwR 临床样本临床样本纵向的圆形组合研究.血等离子体是什么?血液中的血清是血清.

更多相关视频

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

相关概念视频

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...