阿斯伯吉illus terreus 变体 TB21 湿生物质通过现场转化优化,用于生产生物柴油
Rashmi K Bed1, V Ravi Kumar2, Ameeta RaviKumar3
1Department of Biotechnology, Savitribai Phule Pune University, Ganeshkhind Road, Pune, 411007, India.
AMB Express
|February 7, 2025
概括
一种突变的真菌,Aspergillus terreus TB21,显著提高了从甘包水解盐中生产的生物柴油. 这种优化的工艺产生了符合国际标准的高质量的脂肪酸甲基 (FAME).
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 油性真菌是生物柴油生产的有希望的来源.
- Aspergillus terreus可以被基因改造,以改善脂质的积累.
- 甘包水解剂 (SCBH) 为微生物培养提供了低成本的基质.
研究的目的:
- 为了隔离和表征一个高脂产品的变种Aspergillus terreus.
- 为了优化真菌湿生物质到生物柴油的直接现场转化.
- 评估生产的生物柴油的质量和潜力.
主要方法:
- 化学突变的Aspergillus terreus获得TB21的变种.
- 在SCBH.上培育TB21变体.
- 使用实验的统计设计优化的一步 in-situ 酸转化.
- 脂肪酸甲基 (FAME) 含量,生产率,产量和物理化学性质的分析.
主要成果:
- 与野生类型 (49.8%) 相比,TB21变种的脂质含量显著更高 (78.1%).
- 经过优化的转化,FAME从湿生物质中产生了76.5%,从干生物质中产生了38.1%.
- 生产的生物柴油符合国际标准 (ASTM D6751,EN 14214) 和印度标准 (IS 15607).
- 实现了高FAME生产率 (0.55小时−1) 和收益率 (66gL−1).
结论:
- 阿斯珀吉勒斯土壤菌的TB21变体是生物柴油生产的高效微生物资源.
- 湿生物质的直接一 in-situ 转化是一种可行的,高效的生物柴油合成方法.
- 来自SCBH种植的TB21生物质的生物柴油显示出出色的质量和大规模应用的潜力.
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